DNA Barcoding of Sigmodontine Rodents: Identifying Wildlife Reservoirs of Zoonoses

dc.creatorMüller, Livia
dc.creatorGonçalves, Gislene L.
dc.creatorEstrela, Pedro Cordeiro
dc.creatorMarinho, Jorge R.
dc.creatorAlthofi, Sérgio L.
dc.creatorTestoni, André F.
dc.creatorGonzález, Enrique M.
dc.creatorFreitas, Thales R. O.
dc.date2015-08-19T13:49:38Z
dc.date2015-08-19T13:49:38Z
dc.date2013
dc.date.accessioned2023-09-26T20:18:13Z
dc.date.available2023-09-26T20:18:13Z
dc.identifierMÜLLER, Livia; et al. DNA Barcoding of Sigmodontine Rodents: Identifying Wildlife Reservoirs of Zoonoses. Plos One, v.8, n.11, 12p, nov. 2013.
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/11594
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8853758
dc.descriptionSpecies identification through DNA barcoding is a tool to be added to taxonomic procedures, once it has been validated. Applying barcoding techniques in public health would aid in the identification and correct delimitation of the distribution of rodents from the subfamily Sigmodontinae. These rodents are reservoirs of etiological agents of zoonoses including arenaviruses, hantaviruses, Chagas disease and leishmaniasis. In this study we compared distance-based and probabilistic phylogenetic inference methods to evaluate the performance of cytochrome c oxidase subunit I (COI) in sigmodontine identification. A total of 130 sequences from 21 field-trapped species (13 genera), mainly from southern Brazil, were generated and analyzed, together with 58 GenBank sequences (24 species; 10 genera). Preliminary analysis revealed a 9.5% rate of misidentifications in the field, mainly of juveniles, which were reclassified after examination of external morphological characters and chromosome numbers. Distance and model-based methods of tree reconstruction retrieved similar topologies and monophyly for most species. Kernel density estimation of the distance distribution showed a clear barcoding gap with overlapping of intraspecific and interspecific densities < 1% and 21 species with mean intraspecific distance < 2%. Five species that are reservoirs of hantaviruses could be identified through DNA barcodes. Additionally, we provide information for the description of a putative new species, as well as the first COI sequence of the recently described genus Drymoreomys. The data also indicated an expansion of the distribution of Calomys tener. We emphasize that DNA barcoding should be used in combination with other taxonomic and systematic procedures in an integrative framework and based on properly identified museum collections, to improve identification procedures, especially in epidemiological surveillance and ecological assessments.
dc.formatapplication/pdf
dc.languageeng
dc.publisherPlos One
dc.rightsopen access
dc.subjectZoonoses
dc.subjectWildlife Reservoirs
dc.subjectSigmodontine Rodents
dc.subjectDNA Barcoding
dc.subjectCódigo de Barras de DNA Taxonômico
dc.subjectRoedores
dc.subjectZoonose
dc.titleDNA Barcoding of Sigmodontine Rodents: Identifying Wildlife Reservoirs of Zoonoses
dc.typeArticle


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