Evaluation of a rapid serological test for leprosy classification using human serum albumin as the antigen carrier

dc.creatorMoura, Rodrigo Scaliante
dc.creatorPenna, Gerson Oliveira
dc.creatorFujiwara, Tsuyoshi
dc.creatorPontes, Maria Araci de Andrade
dc.creatorCruz, Rossilene
dc.creatorGonçalves, Heitor de Sá
dc.creatorPenna, Maria Lucia Fernandes
dc.creatorCardoso, Ludimila Paula Vaz
dc.creatorStefani, Mariane Martins de Araújo
dc.creatorBührer-Sékula, Samira
dc.date2020-05-25T13:06:30Z
dc.date2020-05-25T13:06:30Z
dc.date2014
dc.date.accessioned2023-09-26T20:17:33Z
dc.date.available2023-09-26T20:17:33Z
dc.identifierMOURA, Rodrigo Scaliante et al. Evaluation of a rapid serological test for leprosy classification using human serum albumin as the antigen carrier. Journal of Immunological Methods, [s.l], v. 412, p. 35-41, oct. 2014.
dc.identifier0022-1759
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/41370
dc.identifier10.1016/j.jim.2014.06.014
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8853481
dc.descriptionGerson Oliveira Penna - Fundação Oswaldo Cruz. Fiocruz Brasília. Brasília, DF, Brasil. Documento produzido em parceria ou por autor vinculado à Fiocruz, mas não consta à informação no documento.
dc.descriptionCAPES / CNPq / DECIT-MS
dc.descriptionThe presence of anti-BSA antibodies may interfere in serological tests, as ELISA or immunochromatographic assays. BSA is frequently used as a blocking agent or as "inert" carrier of antigens, such as the NT-P-BSA, the semi-synthetic trisaccharide analogue of the PGL-I (phenolic glycolipid-I) antigen from the cell wall of the Mycobacterium leprae. PGL-I was prepared and linked to human serum albumin based in the hypothesis that replacing BSA by a human protein carrier would enhance the performance of leprosy serological tests. A total of 1162 serum samples were tested by ELISA and by the ML Flow rapid test using NT-P-BSA or NT-P-HSA antigens. When grouping leprosy patients as paucibacillary (PB) or multibacillary (MB) according to the Ridley & Jopling classification, ML Flow BSA and ML Flow HSA tests correctly allocated 70.9% and 68.6% of patients in the PB group, and 87% and 81% of patients in the MB group, respectively. Concordant results were found in 82.0% (953/1162) (kappa value=0.637; sd=0.023) of samples between ML Flow tests and 85.7% (996/1162) (kappa value=0.703; sd=0.021) between ELISA tests. ML Flow results were statistically similar and the same was true for ELISA tests using HSA or BSA. However, we noticed a tendency to decreased capacity to detect MB patients and an increased positivity among PB patients, HHC, TB patients and healthy controls by the HSA carrier in both ML Flow and ELISA. The PGL-I serology performed by the ML Flow test with BSA or HSA as antigen carriers can be a useful, friendly auxiliary tool to identify patients with higher bacterial load.
dc.description2024-12-31
dc.formatapplication/pdf
dc.languageeng
dc.publisherElsevier
dc.rightsrestricted access
dc.subjectAntibodies, Bacterial
dc.subjectAntigens, Bacterial
dc.subjectBacterial Load
dc.subjectCattle
dc.subjectChromatography, Affinity
dc.subjectEnzyme-Linked Immunosorbent Assay
dc.subjectFalse Positive Reactions
dc.subjectGlycolipids
dc.subjectImmunoglobulin M
dc.subjectLeprosy
dc.subjectMycobacterium leprae
dc.subjectSerologic Tests
dc.subjectSerum Albumin
dc.subjectSerum Albumin, Bovine
dc.subjectLeprosy
dc.subjectBSA
dc.subjectSerology
dc.subjectHanseníase
dc.subjectMycobacterium leprae
dc.subjectTestes Sorológicos
dc.subjectAnticorpos Antibacterianos
dc.subjectAntígenos de Bactérias
dc.subjectAlbumina Sérica
dc.titleEvaluation of a rapid serological test for leprosy classification using human serum albumin as the antigen carrier
dc.typeArticle


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