Fungal extracellular vesicles are involved in intraspecies intracellular communication

dc.creatorBitencourt, Tamires A.
dc.creatorHatanaka, Otavio
dc.creatorPessoni, Andre M.
dc.creatorFreitas, Mateus S.
dc.creatorTrentin, Gabriel
dc.creatorSantos, Patrick
dc.creatorRossi, Antonio
dc.creatorMartinez-Rossi, Nilce M.
dc.creatorAlves, Lysangela Ronalte
dc.creatorCasadevall, Arturo
dc.creatorRodrigues, Marcio Lourenço
dc.creatorAlmeida, Fausto
dc.date2022-02-21T19:17:16Z
dc.date2022-02-21T19:17:16Z
dc.date2022
dc.date.accessioned2023-09-26T20:17:25Z
dc.date.available2023-09-26T20:17:25Z
dc.identifierBITENCOURT, Tamires A. et al. Fungal extracellular vesicles are involved in intraspecies intracellular communication. Mbios, v.13, n. 1, p. 1–14, 2022.
dc.identifier2150-7511
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/51348
dc.identifier2150-7511
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8853424
dc.descriptionFungal infections are associated with high mortality rates in humans. The risk of fungal diseases creates the urgent need to broaden the knowledge base regarding their pathophysiology. In this sense, the role of extracellular vesicles (EVs) has been described to convey biological information and participate in the fungus-host interaction process. We hypothesized that fungal EVs work as an additional element in the communication routes regulating fungal responses in intraspecies interaction systems. In this respect, the aim of this study was to address the gene regulation profiles prompted by fungal EVs in intraspecies recipient cells. Our data demonstrated the intraspecies uptake of EVs in pathogenic fungi, such as Candida albicans, Aspergillus fumigatus, and Paracoccidioides brasiliensis, and the effects triggered by EVs in fungal cells. In C. albicans, we evaluated the involvement of EVs in the yeast-to-hypha transition, while in P. brasiliensis and A. fumigatus the function of EVs as stress transducers was investigated. P. brasiliensis and A. fumigatus were exposed to an inhibitor of glycosylation or UV light, respectively. The results demonstrated the role of EVs in regulating the expression of target genes and triggering phenotypic changes. The EVs treatment induced cellular proliferation and boosted the yeast to hyphal transition in C. albicans, while they enhanced stress responsiveness in A. fumigatus and P. brasiliensis, establishing a role for EVs in fungal intraspecies communication. Thus, EVs regulate fungal behavior, acting as potent message effectors, and understanding their effects and mechanism(s) of action could be exploited in antifungal therapies. Here, we report a study about extracellular vesicles (EVs) as communication mediators in fungi. Our results demonstrated the role of EVs from Candida albicans, Aspergillus fumigatus, and Paracoccidioides brasiliensis regulating the expression of target genes and phenotypic features. We asked whether fungal EVs play a role as message effectors. We show that fungal EVs are involved in fungal interaction systems as potent message effectors, and understanding their effects and mechanisms of action could be exploited in antifungal therapies.
dc.formatapplication/pdf
dc.languagepor
dc.publisherAmerican Society for Microbiology
dc.rightsopen access
dc.subjectAspergillus fumigatus
dc.subjectCandida albicans
dc.subjectParacoccidioides brasiliensis
dc.subjectFungal Infections
dc.subjectExtracellular Vesicles
dc.subjectCell Communication
dc.subjectVesículas Extracelulares
dc.subjectComunicación Celular
dc.subjectVésicules extracellulaires
dc.subjectCommunication cellulaire
dc.subjectInfecções Fúngicas
dc.subjectVesículas Extracelulares
dc.subjectComunicação Celular
dc.titleFungal extracellular vesicles are involved in intraspecies intracellular communication
dc.typeArticle


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