Immunostimulatory activity of ConBr: a focus on splenocyte proliferation and proliferative cytokine secretion

dc.creatorSilva, Flávio de Oliveira
dc.creatorSantos, Priscila das Neves
dc.creatorMelo, Cristiane Moutinho Lagos de
dc.creatorTeixeira, Edson Holanda
dc.creatorCavada, Benildo de Sousa
dc.creatorArruda, Francisco Vassiliepe Sousa
dc.creatorCajazeiras, João Batista
dc.creatorAlmeida, Alysson Chaves
dc.creatorPereira, Valéria Alves Rêgo
dc.creatorPorto, Ana Lúcia Figueiredo
dc.date2018-09-05T12:39:05Z
dc.date2018-09-05T12:39:05Z
dc.date2011
dc.date.accessioned2023-09-26T20:17:13Z
dc.date.available2023-09-26T20:17:13Z
dc.identifierDE OLIVEIRA SILVA, F. et al. Immunostimulatory activity of ConBr: a focus on splenocyte proliferation and proliferative cytokine secretion. Cell and Tissue Research, v. 346, n. 2, p. 237–244, nov. 2011.
dc.identifier1432-0878
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/28565
dc.identifier10.1007/s00441-011-1239-x
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8853356
dc.descriptionFundação de Amparo à Pesquisa de Pessoal de Nível Superior da CAPES (Fundação de Amparo à Ciência e Tecnologia do Estado de Pernambuco, processo APQ-0493-2.11 / 08) e do Centro de Pesquisas Aggeu Magalhães. Fundação Oswaldo Cruz em Recife, Brasil (CPqAM / FIOCRUZ).
dc.descriptionLectins constitute a class of glycoproteins, which are capable of selectively and reversibly binding to carbohydrates, distinguishing small structural differences in complex oligosaccharides. Studies have shown that the binding of lectins to cell-surface carbohydrates can lead to various effects such as cellular proliferation, histamine release and cytokine production. Canavalia brasiliensis lectin (ConBr) is a (D-mannose) D-glucose lectin. In this study, murine splenocytes were cultured to determine the effect of ConBr on cell proliferation, nitric oxide (NO) release and cytokine secretion. In addition, cellular viability assays were performed to evaluate any mitogenic activity induced by this lectin. ConBr significantly increased cell proliferation with minimal cell damage. This lectin was able to induce an increased production of cytokines such as IL-2, IL-6 and IFN-γ and a decreased production of IL- 10. The release of NO was also observed. The results of this study indicate that ConBr could potentially be used as an immunomodulator.
dc.description2050-01-01
dc.formatapplication/pdf
dc.languageeng
dc.publisherSpringer-Verlag
dc.rightsrestricted access
dc.subjectCanavalia brasiliensis
dc.subjectConBr
dc.subjectLectinas
dc.subjectGlucose / lectina de ligação à manose
dc.subjectAtividade proliferativa
dc.subjectCanavalia brasiliensis
dc.subjectConBr
dc.subjectLectins
dc.subjectGlucose/mannose binding-lectin
dc.subjectProliferative activity
dc.subjectAdjuvantes Imunológicos / Farmacologia
dc.subjectCélulas Cultivadas
dc.subjectProliferação Celular / efeitos de drogas
dc.subjectSobrevivência Celular / efeitos de drogas
dc.subjectConcanavalina A / farmacologia
dc.subjectCitocinas / secreção
dc.subjectImunização
dc.subjectInterferon gama / biossíntese
dc.subjectRatos, endogâmicos BALB C
dc.subjectÓxido Nítrico / secreção
dc.subjectLectinas vegetais / farmacologia
dc.subjectBaço / citologia
dc.subjectBaço / efeitos de drogas
dc.subjectBaço / secreção
dc.titleImmunostimulatory activity of ConBr: a focus on splenocyte proliferation and proliferative cytokine secretion
dc.typeArticle


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