An experimental protocol for the establishment of dogs with long-term cellular immune reactions to Leishmania antigens

dc.creatorTeixeira, Márcia Cristina Aquino
dc.creatorOliveira, Geraldo Gileno de Sá
dc.creatorSantos, Patrícia Oliveira Meira
dc.creatorBahiense, Thiago Campanharo
dc.creatorSilva, Virgínia Maria Goes da
dc.creatorRodrigues, Márcio Silva
dc.creatorLarangeira, Daniela Farias
dc.creatordosSantos, Washington Luis Conrado
dc.creatorPontes-de-Carvalho, Lain Carlos
dc.date2012-12-20T19:35:38Z
dc.date2012-12-20T19:35:38Z
dc.date2011
dc.date.accessioned2023-09-26T20:16:51Z
dc.date.available2023-09-26T20:16:51Z
dc.identifierTEIXEIRA, M. C. A. et al. An experimental protocol for the establishment of dogs with long-term cellular immune reactions to Leishmania antigens. Memórias do Instituto Oswaldo Cruz, v. 106, n. 2, p. 182-189, mar. 2011.
dc.identifier0074-0276
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/6049
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8853208
dc.descriptionDomestic dogs are considered to be the main reservoirs of zoonotic visceral leishmaniasis. In this work, we evaluated a protocol to induce Leishmania infantum/Leishmania chagasi-specific cellular and humoral immune responses in dogs, which consisted of two injections of Leishmania promastigote lysate followed by a subcutaneous inoculation of viable promastigotes. The primary objective was to establish a canine experimental model to provide positive controls for testing immune responses to Leishmania in laboratory conditions. After inoculation of viable promastigotes, specific proliferative responses of peripheral blood mononuclear cells (PBMCs) to either Leishmania lysate or recombinant proteins, the in vitro production of interferon-γ by antigen-stimulated PBMCs and a significant increase in circulating levels of anti-Leishmania antibodies were observed. The immunized dogs also displayed positive delayed-type hypersensitivity reactions to Leishmania crude antigens and to purified recombinant proteins. An important finding that supports the suitability of the dogs as positive controls is that they remained healthy for the entire observation period, i.e., more than seven years after infection. Following the Leishmania antigen lysate injections, the infection of dogs by the subcutaneous route appears to induce a sustained cellular immune response, leading to an asymptomatic infection. This provides a useful model for both the selection of immunogenic Leishmania antigens and for immunobiological studies on their possible immunoprotective activities.
dc.formatapplication/pdf
dc.languageeng
dc.rightsopen access
dc.subjectLeishmania infantum
dc.subjectLeishmania chagasi
dc.subjectDogs
dc.subjectImmunization
dc.subjectCellular immune response
dc.subjectAnticorpos Antiprotozoários/sangue
dc.subjectAntígenos de Protozoários/imunologia
dc.subjectDoenças do Cão/imunologia
dc.subjectImunidade Celular/imunologia
dc.subjectLeishmania infantum/imunologia
dc.subjectLeishmaniose Visceral/veterinária
dc.subjectAnticorpos Antiprotozoários/imunologia
dc.subjectProliferação de Células
dc.subjectELISA
dc.subjectHipersensibilidade Tardia/imunologia
dc.subjectInterferon gama/sangue
dc.subjectInterferon gama/imunologia
dc.subjectLeishmania infantum
dc.subjectLeishmaniose Visceral/imunologia
dc.subjectAtivação Linfocítica/imunologia
dc.subjectModelos Animais
dc.subjectFatores de Tempo
dc.subjectAnimais
dc.subjectCães
dc.subjectResearch Support, Non-U.S. Gov't
dc.titleAn experimental protocol for the establishment of dogs with long-term cellular immune reactions to Leishmania antigens
dc.typeArticle


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