Primary culture of the region of the amebocyte-producing organ of the snail Biomphalaria glabrata, the intermediate host of Schistosoma mansoni

dc.creatorBarbosa, Lúcio Macedo
dc.creatorSilva, L. M.
dc.creatorCoelho, Paulo Marcos Zach
dc.creatorSantos, S. R.
dc.creatorFortes Dias, Consuelo Latorre
dc.date2013-05-13T14:42:30Z
dc.date2013-05-13T14:42:30Z
dc.date2006
dc.date.accessioned2023-09-26T20:16:02Z
dc.date.available2023-09-26T20:16:02Z
dc.identifierBARBOSA, L; et al. Primary culture of the region of the amebocyte-producing organ of the snail Biomphalaria glabrata, the intermediate host of Schistosoma mansoni. Mem Inst Oswaldo Cruz, Rio de Janeiro, Vol. 101(6): 639-643, 2006
dc.identifier0074-0276
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/6486
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8852878
dc.descriptionFundação Ezequiel Dias, Centro de Pesquisas René-Rachou, CNPq
dc.descriptionBiomphalaria glabrata snails are major hosts for the digenetic trematoda Schistosoma mansoni, the causative agent of human schistosomiasis. The success or failure of the infection will be dependent on the mobilization of the molluskan internal defense system, where a major role will be played by circulating hemocytes produced by the APO (amebocyte-producing organ) of the snail. In this report, the primary culture of the APO region of B. glabrata was obtained for the first time, as well as a control culture of the ovotestis. Three different cell populations migrated easily from the explants in culture, with no need of any dispersion agent. The cells grew in suspension at anincubation temperature of 15ºC and the cultures were maintained viable for up to two weeks. Two of these cell populations obtained resembled cell types known to be present in the hemolymph of Biomphalaria. The availabilityof APO cells in culture may contribute to a better understanding of the internal defense in mollusks, in general, as well as the specific response of B. glabrata to S. mansoni infection.
dc.formatapplication/pdf
dc.languageeng
dc.rightsopen access
dc.subjectBiomphalaria
dc.subjectschistosomiasis
dc.subjectSchistosoma mansoni
dc.subjecthemocyte
dc.subjecttissue culture
dc.titlePrimary culture of the region of the amebocyte-producing organ of the snail Biomphalaria glabrata, the intermediate host of Schistosoma mansoni
dc.typeArticle


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