Frequency, active infection and load of Leishmania infantum and associated histological alterations in the genital tract of male and female dogs

dc.creatorBoechat, Viviane Cardoso
dc.creatorPereira, Sandro Antonio
dc.creatorMendes Júnior, Artur Augusto Velho
dc.creatorSantos, Shanna Araujo dos
dc.creatorMiranda, Luciana de Freitas Campos
dc.creatorFigueiredo, Fabiano Borges
dc.creatorFerreira, Luiz Claudio
dc.creatorRodrigues, Francisco das Chagas de Carvalho
dc.creatorOliveira, Raquel de Vasconcellos Carvalhaes de
dc.creatorTeles -de-Freitas, Rayane
dc.creatorBruno, Rafaela Vieira
dc.creatorMorgado, Fernanda Nazaré
dc.creatorMenezes, Rodrigo Caldas
dc.date2020-09-22T20:24:52Z
dc.date2020-09-22T20:24:52Z
dc.date2020
dc.date.accessioned2023-09-26T20:15:30Z
dc.date.available2023-09-26T20:15:30Z
dc.identifierBOECHAT, Viviane Cardoso et al. Frequency, active infection and load of Leishmania infantum and associated histological alterations in the genital tract of male and female dog. PLoS One, v. 15, n. 9, p. 1-18, 2020.
dc.identifier1932-6203
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/43558
dc.identifier10.1371/journal.pone.0238188
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8852659
dc.descriptionVisceral leishmaniasis caused by the protozoan Leishmania infantum is a zoonosis. The domestic dog is the primary reservoir in urban areas. This study aimed to evaluate the frequency, active infection and load of L. infantum in the genital tract of male and female dogs seropositive for this parasite, as well as to identify histological genital alterations associated with this protozoan. We studied 45 male and 25 female L. infantum-seropositive non castrated dogs from the same endemic area in Brazil. Tissue samples from the testis, epididymis, prostate, vulva, vagina, and uterus were examined by singleplex qPCR and parasitological tests (histopathology, immunohistochemistry, and parasitological culture). The latter were performed for the detection of active infection (parasites able to multiply and to induce lesions). Forty-four (98%) males and 25 (100%) females were positive for L. infantum in the genital tract (epididymis: 98%; vulva: 92%; vagina: 92%; testis: 91%; uterus: 84%; prostate: 66%). Active infection in the genital tract was confirmed in 69% of males and 64% of females (32% in the uterus). Parasite loads were similar in the testis, vulva, epididymis and vagina and lower in the prostate. Only the parasite load in the vagina was significantly associated with the number of clinical signs. Granulomatous inflammation predominated in all organs, except for the prostate. Only in the testis and epididymis was the inflammatory infiltrate significantly more intense among dogs with a higher parasite load in these organs. The high frequency, detection of active infection and similarity of L. infantum loads in the genital tract of infected males and females suggest the potential of venereal transmission of this parasite by both sexes and of vertical transmission by females in the area studied. Additionally, vertical transmission may be frequent since active L. infantum infection was a common observation in the uterus.
dc.formatapplication/pdf
dc.languagepor
dc.publisherPublic Library of Science
dc.rightsopen access
dc.subjectLeishmaniasis, Visceral
dc.subjectDogs
dc.subjectReproductive Tract Infections
dc.subjectInfectious Disease Transmission, Vertical
dc.subjectLeishmaniasis Visceral
dc.subjectPerros
dc.subjectInfecciones del Sistema Genital
dc.subjectTransmisión Vertical de Enfermedad Infecciosa
dc.subjectLeishmaniose viscérale
dc.subjectChiens
dc.subjectInfections de l'appareil reproducteur
dc.subjectTransmission verticale de maladie infectieuse
dc.subjectLeishmaniose Visceral
dc.subjectCães
dc.subjectInfecções do Sistema Genital
dc.subjectTransmissão Vertical de Doença Infecciosa
dc.titleFrequency, active infection and load of Leishmania infantum and associated histological alterations in the genital tract of male and female dogs
dc.typeArticle


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