Ultrastructural and enzymatic alterations in the ovary of Rhodnius prolixus infected with Trypanosoma rangeli

dc.creatorMachado, Guilherme S.
dc.creatorLopes, Rosane L.
dc.creatorOliveira, Simone S. C.
dc.creatorFreitas, Simone P. C.
dc.creatorSantos-Mallet, Jacenir R.
dc.creatorSantos, André L. S.
dc.creatorFeder, Denise
dc.creatorGomes, Suzete A. O.
dc.date2022-05-06T19:32:29Z
dc.date2022-05-06T19:32:29Z
dc.date2022
dc.date.accessioned2023-09-26T20:15:10Z
dc.date.available2023-09-26T20:15:10Z
dc.identifierMACHADO, Guilherme S. et al. Ultrastructural and enzymatic alterations in the ovary of Rhodnius prolixus infected with Trypanosoma rangeli. Asian Pacific Journal of Tropical Medicine, v. 15, n. 2, p. 71- 78, May 2022.
dc.identifier2352-4146
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/52577
dc.identifier10.4103/1995-7645.338437
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8852516
dc.descriptionTo investigate the morphological structure of ovarian follicular cells and biochemical parameters of both ovaries and fat bodies (sites of vitellogenesis) from Rhodnius (R.) prolixus infected with Trypanosoma (T.) rangeli. Methods: Adult virgin females of R. prolixus were fed upon a membrane apparatus containing heat-inactivated citrated rabbit blood and a suspension of T. rangeli epimastigotes (Macias strain). Females from the control group and all the males received parasitefree blood. Transmission electron microscopy was used to reveal the morphological aspects of ovarian follicle cells in both control and parasite-infected groups. Protein profile, proteolytic activities and Western blotting analyses were performed in either ovary or fat body samples of control and parasite-infected groups. Results: According to the ultrastructural data, T. rangeli infection elicited a degeneration process in the ovarian follicular cells of R. prolixus. Proteolytic assays indicated a reduction in the activity of aspartic peptidases in the ovary and fat body from parasite-infected group, while a significant increase in the cysteine peptidase activity was measured in both insect organs. Additionally, immunoblotting revealed that vitellogenin was overexpressed in the ovary of parasite-infected insects. Conclusions: T. rangeli infection seems to elicit an early programmed cell death in the ovarian follicle cells as well as induces the modulation on the activities of different peptidase classes in either ovaries or fat bodies and the overexpression of the vitellogenin in the ovary of R. prolixus.
dc.formatapplication/pdf
dc.languageeng
dc.publisherScienceDirect.com by Elsevier
dc.rightsopen access
dc.subjectTrypanosoma rangeli
dc.subjectRhodnius prolixus
dc.subjectFolículo ovariano
dc.subjectVitelogenina
dc.subjectProtease aspártica
dc.subjectCisteína protease
dc.subjectTrypanosoma rangeli
dc.subjectRhodnius prolixus
dc.subjectOvarian follicle
dc.subjectVitellogenin
dc.subjectAspartic protease
dc.subjectCysteine protease
dc.titleUltrastructural and enzymatic alterations in the ovary of Rhodnius prolixus infected with Trypanosoma rangeli
dc.typeArticle


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