Validation of quantitative real-time PCR for the in vitro assessment of antileishmanial drug activity

dc.creatorGomes, Luciana Inácia
dc.creatorGonzaga, Felipe M.
dc.creatorTeixeira, Eliane de Morais
dc.creatorRodrigues, Bruna Soares de Souza Lima
dc.creatorFreire, Verônica V.
dc.creatorRabello, Ana Lúcia Teles
dc.date2014-03-26T18:48:08Z
dc.date2014-03-26T18:48:08Z
dc.date2012
dc.date.accessioned2023-09-26T20:14:33Z
dc.date.available2023-09-26T20:14:33Z
dc.identifierGOMES, Luciana Inácia et al. Validation of quantitative real-time PCR for the in vitro assessment of antileishmanial drug activity. Experimental Parasitology. 2012, vol.131, pp. 175-179
dc.identifier0014-4894
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/7458
dc.identifier10.1016/j.exppara.2012.03.021
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8852259
dc.descriptionIn vitro assays play an important role in the discovery and development of new antileishmanial drugs. The classic macrophage-amastigote models using murine peritoneal macrophages or human-monocyte derived macrophages as host cells are useful for drug screening. A major limitation of these models is the dependence on microscopic counting, a time-consuming and subjective method of analysis. The present study describes a detailed protocol for applying quantitative real-time PCR (qPCR) as an accurate and sensitive tool to assess parasite load in an amastigote-macrophage model. This assay can be performed in a standardized medium-to-high throughput procedure, replacing traditional readout of number of amastigote per macrophages by DNA load measurement.
dc.formatapplication/pdf
dc.languageeng
dc.publisherElsevier
dc.rightsopen access
dc.subjectantileishmanial drugs
dc.subjectLeishmania
dc.subjectIn vitro assays
dc.subjectQuantitative real-time PCR
dc.titleValidation of quantitative real-time PCR for the in vitro assessment of antileishmanial drug activity
dc.typeArticle


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