Avaliação histológica e imuno-histoquímica da colonização vaginal por Leptospira em vacas com fluido vaginal positivo à PCR*

Histological and immunohistochemical evaluation of Leptospira vaginal colonization in cows with vaginal fluid positive to PCR.

dc.creatorOliveira, Fernanda Santana
dc.creatorOliveira, Daiana de
dc.creatorMartins Filho, Emanoel Ferreira
dc.creatorCosta, Federico
dc.creatorRistow, Paula
dc.creatorPinna, Melissa Hanzen
dc.creatorLilenbaum, Walter
dc.date2017-05-09T18:16:03Z
dc.date2017-05-09T18:16:03Z
dc.date2016
dc.date.accessioned2023-09-26T20:14:09Z
dc.date.available2023-09-26T20:14:09Z
dc.identifierOLIVEIRA, F. S. et al. Avaliação histológica e imuno-histoquímica da colonização vaginal por Leptospira em vacas com fluido vaginal positivo à PCR. Revista Brasileira de Medicina Veterinária, v. 38, Supl.1, p. 163-167, 2016.
dc.identifier1413-0130
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/18802
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8852106
dc.descriptionLeptospirosis in cattle is characterized by causing significant economic losses due to reproductive problems as well as drop in production of meat and milk. Subsequent abortion may be caused by the persistence of the agent in the reproductive tract and thus become a possible transmission source. This study aimed to investigate through histological and immunohistochemical evaluation the vaginal colonization of cows with polymerase chain reaction (PCR) detection of leptospiral DNA in vaginal fluid. The experiment was conducted in two stages: the first identified carrier animals from the PCR in vaginal fluid samples, urine and kidney belonging to 147 cows for the slaughterhouse under official inspection in the state of Rio de Janeiro. The second performed histological processing of vaginal fragments of animals with vaginal fluid positive to PCR, using hematoxylin-eosin (HE) and immunohistochemistry (IHC) techniques. Of the 147 vaginal fluid samples analyzed, 28% (41/147) were positive in PCR and formed the working group for histological analysis. It is emphasized that from these samples, 82.92% (34/41) had the presence of leptospiral DNA in urine and/or kidney, while 17.08% (7/41) were negative for PCR of urine and/or kidney. Nor pathological changes consistent with Leptospira infection and neither the bacteria were identified in the vaginal epithelium through HE and immune-histochemical techniques. Thus, although we found molecular evidence for the presence of leptospiral DNA in vaginal fluid of carrier ani-mals, the presence of the agent in the vagina or changes compatible with infection were not verified. We believe that further studies are needed to assess colonization by Leptospira in the genital tract of cows.
dc.descriptionFAPERJ, CNPq e CAPES
dc.formatapplication/pdf
dc.languagepor
dc.publisherUniversidade Federal Fluminense. Faculdade de Veterinária
dc.rightsopen access
dc.subjectLeptospirose
dc.subjectAparelho genital
dc.subjectDiagnóstico molecular
dc.subjectBovinos
dc.subjectLeptospirosis
dc.subjectGenital tract
dc.subjectMolecular diagnosis
dc.subjectBovines
dc.subjectLeptospirose
dc.subjectSistema genital
dc.subjectDiagnósticos moleculares
dc.subjectBovinos
dc.titleAvaliação histológica e imuno-histoquímica da colonização vaginal por Leptospira em vacas com fluido vaginal positivo à PCR*
dc.titleHistological and immunohistochemical evaluation of Leptospira vaginal colonization in cows with vaginal fluid positive to PCR.
dc.typeArticle


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