Naïve donor responses to Schistosoma mansoni soluble egg antigens.

dc.creatorReis, Eliana Almeida Gomes
dc.creatorCarmo, Theomira Mauadie de Azevedo
dc.creatorMcBride, Alan John Alexander
dc.creatorHarn Junior, Donald A
dc.creatorReis, Mitermayer Galvão dos
dc.date2014-04-25T18:33:00Z
dc.date2014-04-25T18:33:00Z
dc.date2007
dc.date.accessioned2023-09-26T20:13:47Z
dc.date.available2023-09-26T20:13:47Z
dc.identifierREIS, E. A. G. et al. Naïve donor responses to Schistosoma mansoni soluble egg antigens. Scandinavian Journal of Immunology , v.66, n. 6, p. 662–670, 2007.
dc.identifier1365-3083
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/7545
dc.identifier10.1111/j.1365-3083.2007.02024.x
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8851944
dc.descriptionSchistosome infection induces profound Th-biasing and immune suppression. Although much has been examined in mice, few studies have examined responses of naı¨ve humans to schistosome antigens. In this study, we examined the response of naı¨ve human peripheral blood mononuclear cells (nPBMC) to stimulation with Schistosoma mansoni soluble egg antigen (SEA) using a priming in vitro (PIV) assay. We found that SEA induced a pronounced CD4+ T-helper cell response based on cytokine secretion and phenotyping markers. SEA-stimulated nPBMC (SEA cells) at day 7 post-priming and after the first recall consisted predominantly of Th0-like CD4+ T cells. Following the second recall, the majority of donor (10 ⁄ 12) responses were Th2-like. The cell population consisted of approximately 64% CD4+, 17% CD8+high, 12% CD19+, and 7% CD23+ cells. The CD4+ population also expressed HLA-DR+, CD54+, CD45RO+ and CD25+ whereas the CD19+ cells expressed CD80 and CD86. Following priming, we detected high levels of IL-6, IFN-c, IL-12p40, IL-10 and IL-5. Upon restimulation, SEA cells secreted IL-5 and high levels of IL-10, typical of a Th2-like response. The data presented herein shows that the majority of naı¨ve donor dendritic cells, following stimulation with SEA, prime and clonally expand SEA-specific T cells towards a Th2-type response. However, two donors responded with an atypical response, producing IFN-c coincident with low levels of IL-10. Whether this differential response was due to HLA or other genes was not determined but is currently under investigation.
dc.formatapplication/pdf
dc.languageeng
dc.publisherBlackwell Publishing Ltd.
dc.rightsopen access
dc.subjectAntígenos de Helmintos/administração & dosagem
dc.subjectÓvulo/imunologia
dc.subjectSchistosoma mansoni/imunologia
dc.subjectEsquistossomose mansoni/imunologia
dc.subjectAdulto
dc.subjectAnimais
dc.subjectAntígenos de Helmintos/imunologia
dc.subjectLinfócitos T CD4-Positivos/imunologia
dc.subjectCitocinas/imunologia
dc.subjectCélulas Dendríticas/parasitologia
dc.subjectCitometria de Fluxo
dc.subjectHumanos
dc.subjectSolubilidade
dc.subjectCélulas Th2/imunologia
dc.subjectCélulas Th2/parasitologia
dc.titleNaïve donor responses to Schistosoma mansoni soluble egg antigens.
dc.typeArticle


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