The citrus flavonone naringenin reduces lipopolysaccharide-induced inflammatory pain and leukocyte recruitment by inhibiting NF-κB activation

dc.creatorPinho-Ribeiro, Felipe A.
dc.creatorZarpelon, Ana Carla
dc.creatorMizokami, Sandra S.
dc.creatorBorghi, Sergio M.
dc.creatorBordignon, Juliano
dc.creatorSilva, Rangel L.
dc.creatorCunha, Thiago M.
dc.creatorAlves-Filho, Jose C.
dc.creatorCunha, Fernando Q.
dc.creatorCasagrande, Rubia
dc.creatorVerri Jr, Waldiceu Aparecido
dc.date2016-10-13T16:39:00Z
dc.date2016-10-13T16:39:00Z
dc.date2016
dc.date.accessioned2023-09-26T20:13:28Z
dc.date.available2023-09-26T20:13:28Z
dc.identifierPINHO-RIBEIRO, F. A. et al. The citrus flavonone naringenin reduces lipopolysaccharide-induced inflammatory pain and leukocyte recruitment by inhibiting NF-κB activation. Journal of Nutritional Biochemistry, v. 33, p. 8-14, 2016.
dc.identifier0955-2863
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/16223
dc.identifier10.1016/j.jnutbio.2016.03.013
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8851811
dc.descriptionCoordenadoria de Aperfeiçoamento de Pessoal de Nível Superior (CAPES, Brazil), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), São Paulo Research Foundation under grant agreements n° 2011/19670-0 (Thematic project) and 2013/08216-2 (Center for Research in Inflammatory Disease), Ministério da Ciência, Tecnologia e Inovação (MCTI), SETI, Fundação Araucária, and Parana State Government
dc.descriptionLipopolysaccharide (LPS) is the major structural component of Gram-negative bacteria cell wall and a highly pro-inflammatory toxin. Naringenin is found in Citrus fruits and exhibits antioxidant and anti-inflammatory properties through inhibition of NF-κB activation but its effects in LPS-induced inflammatory pain and leukocyte recruitment were not investigated yet. We investigated the effects of naringenin in mechanical hyperalgesia, thermal hyperalgesia and leukocyte recruitment induced by intraplantar injection of LPS in mice. We found that naringenin reduced hyperalgesia to mechanical and thermal stimuli, myeloperoxidase (MPO, a neutrophil and macrophage marker) and N-acetyl-β-D-glucosaminidase (NAG, a macrophage marker) activities, oxidative stress and cytokine (TNF-α, IL-1β, IL-6, and IL-12) production in the paw skin. In the peritoneal cavity, naringenin reduced neutrophil and mononuclear cell recruitment, and abrogated MPO and NAG activity, cytokine and superoxide anion production, and lipid peroxidation. In vitro, pre-treatment with naringenin inhibited superoxide anion and cytokine (TNF-α, IL-1β, IL-6, and IL-12) production by LPS-stimulated RAW 264.7 macrophages. Finally, we demonstrated that naringenin inhibited NF-κB activation in vitro and in vivo. Therefore, naringenin is a promising compound to treat LPS-induced inflammatory pain and leukocyte recruitment.
dc.formatapplication/pdf
dc.languageeng
dc.publisherBernhard Hennig
dc.rightsrestricted access
dc.subjectInflammatory pain
dc.subjectCytokines
dc.subjectOxidative stress
dc.subjectLeukocyte recruitment
dc.subjectNF-κB
dc.subjectCitocinas
dc.subjectEstresse Oxidativo
dc.titleThe citrus flavonone naringenin reduces lipopolysaccharide-induced inflammatory pain and leukocyte recruitment by inhibiting NF-κB activation
dc.typeArticle


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