Proteins involved on TGF-β pathway are up-regulated during the acute phase of experimental Chagas disease

dc.creatorFerreira, Roberto Rodrigues
dc.creatorSouza, Elen Mello de
dc.creatorOliveira, Fabiane Loiola de
dc.creatorFerrão, Patrícia Mello
dc.creatorGomes, Leonardo Henrique Ferreira
dc.creatorLIma, Leila Mendonça
dc.creatorBatista, Marcelo Meuser
dc.creatorBailly, Sabine
dc.creatorFeige, Jean Jacques
dc.creatorAraujo-Jorge, Tania C.
dc.creatorWaghabi, Mariana Caldas
dc.date2016-07-05T16:08:11Z
dc.date2016-07-08T12:22:01Z
dc.date2016-07-05T16:08:11Z
dc.date2016-07-08T12:22:01Z
dc.date2016
dc.date.accessioned2023-09-26T20:12:52Z
dc.date.available2023-09-26T20:12:52Z
dc.identifierFERREIRA, Roberto Rodrigues; et al. Proteins involved on TGF-β pathway are up-regulated during the acute phase of experimental Chagas disease. Immunobiology, v.221, n.5, p.587-594, May 2016.
dc.identifier0171-2985
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/14780
dc.identifier10.1016/j.imbio.2016.01.009
dc.identifier1878-3279
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8851557
dc.descriptionStudies developed by our group in the last years have shown the involvement of TGF-β in acute and chronic Chagas heart disease, with elevated plasma levels and activated TGF-β cell signaling pathway as remarkable features of patients in the advanced stages of this disease, when high levels of cardiac fibrosis is present. Imbalance in synthesis and degradation of extracellular matrix components is the basis of pathological fibrosis and TGF-β is considered as one of the key regulators of this process. In the present study, we investigated the activity of the TGF-β signaling pathway, including receptors and signaling proteins activation in the heart of animals experimentally infected with Trypanosoma cruzi during the period that mimics the acute phase of Chagas disease. We observed that T. cruzi—infected animals presented increased expression of TGF-β receptors. Overexpression of receptors was followed by an increased phosphorylation of Smad2/3, p38 and ERK. Furthermore, we correlated these activities with cellular factors involved in the fibrotic process induced by TGF-β. We observed that the expression of collagen I, fibronectin and CTGF were increased in the heart of infected animals on day 15 post-infection. Correlated with the increased TGF-β activity in the heart, we found that serum levels of total TGF-β were significantly higher during acute infection. Taken together, our data suggest that the commitment of the heart associates with increased activity of TGF-β pathway and expression of its main components. Our results, confirm the importance of this cytokine in the development and maintenance of cardiac damage caused by T. cruzi infection.
dc.formatapplication/pdf
dc.languageeng
dc.publisherScienceDirect
dc.rightsrestricted access
dc.subjectTrypanosoma cruzi
dc.subjectDoença de Chagas
dc.subjectInfecção
dc.subjectFibrose
dc.subjectTrypanosoma cruzi
dc.subjectTGF-β
dc.subjectInfection
dc.subjectChagas Disease
dc.subjectFibrosis
dc.titleProteins involved on TGF-β pathway are up-regulated during the acute phase of experimental Chagas disease
dc.typeArticle


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