Plasmodium berghei: cerebral malaria in CBA mice is not clearly related to plasma TNF levels or intensity of histopathological changes

dc.creatorCarvalho, L. J. M.
dc.creatorLenzi, Henrique Leonel
dc.creatorMachado, Marcelo Pelajo
dc.creatorOliveira, Denise N.
dc.creatorDaniel-Ribeiro, Cláudio Tadeu
dc.creatorCruz, Maria de Fátima Ferreira da
dc.date2012-11-26T14:26:53Z
dc.date2012-11-26T14:26:53Z
dc.date2000
dc.date.accessioned2023-09-26T20:12:46Z
dc.date.available2023-09-26T20:12:46Z
dc.identifierCARVALHO, L. J. M. et al. Plasmodium berghei: cerebral malaria in CBA mice is not cleary related to plasma TNF levels or intensity of histopathological changes. Experimental Parasitology, v. 95, n.1, p. 1-7, 2000.
dc.identifier0014-4894
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/5869
dc.identifierhttp://dx.doi.org/10.1006/expr.2000.4508
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8851517
dc.descriptionThe authors dedicate this paper in honor of the Instituto Oswaldo Cruz on the occasion of the Centenary of its foundation, May 25th, 1900. The authors are grateful to Bruno Silva Vale and Arturo Max Arana-Pino for processing images in scanning light confocal microscopy, to Isaac Lima da Silva Filho and Janete Cuba from Evandro Chagas Hospital–IOC–Fiocruz for hematological analysis, to Belmira Ferreira dos Santos from the Fiocruz Central Animal House for providing us with CBA/J mouse strain and to Dr. Heitor Franco Jr. (IMT–São Paulo, Brazil) for kindly providing the P. berghei ANKA strain. This work was supported by funds of the Conselho Nacional de Desenvolvimento Tecnológico (CNPq) and FAPERJ–Brazil.
dc.descriptionPlasmodium berghei ANKA infection in CBA/J mice leads to the development of cerebral malaria (CM) that kills 80–90% of the animals in 6–9 days. This model has been used to study the pathogenesis of CM, which is a major cause of morbidity and mortality in Plasmodium falciparum-infected individuals. The role of cytokines in the induction of CM in the murine model has been well documented, but most studies have been restricted to the peak of neurological manifestations. Here we used a sequential approach to compare mice that developed CM with those that developed no cerebral pathology. Animals were examined for systemic histopathological changes and plasma Tumor Necrosis Factor-α (TNF) levels. The objectives were (a) to further determine the importance of factors commonly associated with murine CM—such as elevated levels of TNF and the presence of hemorrhage and vascular plugging—by comparing mice at different stages of infection and/or with different outcomes following infection and (b) to examine the importance of systemic changes—course of parasitemia and histopathological alterations in brain, liver, and lungs—in the development of CM. The data suggest that (a) the clinical manifestation of CM appears to be associated with a wave of merozoite release on days 6–7, (b) murine CM does not present reliable histopathological indicators, (c) there is no topographic association between the occurrence of intravascular plugging and the hemorrhagic foci, (d) monocyte–monocyte and monocyte–endothelial cell adherence were the most expressive histopathological events and were not restricted to brain vessels, (e) blood levels of TNF are not indicative of the local tissue reaction, (f) adhesiveness of monocyte/endothelial cells fluctuate during infection and is dissociated from the lymphocyte homing to the liver, and (g) pulmonary megakaryocytosis (megakaryopoiesis?) is a late event in the lungs.
dc.formatapplication/pdf
dc.languageeng
dc.publisherElsevier
dc.rightsopen access
dc.subjectcerebral malaria (CM)
dc.subjectlasmodium berghei ANKA
dc.subjectCBA/J mice
dc.subjectumor Necrosis Factor-α (TNF)
dc.subjectcytokines
dc.subjectmerozoite release
dc.subjecthemorrhages
dc.subjecthistopathology
dc.subjectintercellular cell adhesion molecule-1 (ICAM-1)
dc.subjectlymphocyte function antigen-1 (LFA-1)
dc.subjectEncéfalo
dc.subjectFígado
dc.subjectLung
dc.subjectMalária Cerebral
dc.subjectPlasmodium berghei
dc.subjectFator de Necrose Tumoral alfa
dc.titlePlasmodium berghei: cerebral malaria in CBA mice is not clearly related to plasma TNF levels or intensity of histopathological changes
dc.typeArticle


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