Retrovirus infections in a sample of injecting drug users in Rio de Janeiro City, Brazil: prevalence of HIV-1 subtypes, and co-infection with HTLV-I/II.

dc.creatorGuimarães, Monick Lindenmeyer
dc.creatorBastos, Francisco Inácio Pinkusfeld Monteiro
dc.creatorTelles, Paulo R
dc.creatorCastro Filho, Bernardo Galvão
dc.creatorDiaz, Ricardo Sobhie
dc.creatorBongertz, Vera
dc.creatorMorgado, Mariza Gonçalves
dc.date2014-08-07T12:26:46Z
dc.date2014-08-07T12:26:46Z
dc.date2001
dc.date.accessioned2023-09-26T20:12:45Z
dc.date.available2023-09-26T20:12:45Z
dc.identifierGUIMARÃES, M. L. et al. Retrovirus infections in a sample of injecting drug users in Rio de Janeiro City, Brazil: prevalence of HIV-1 subtypes, and co-infection with HTLV-I/II. Journal of Clinical Virology, v. 21, n. 2, p. 143–151, 2001.
dc.identifier1386-6532
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/8147
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8851510
dc.descriptionBACKGROUND: Retrovirus infections among injecting drug users (IDUs), a core at-risk population for both HIV-1 and HTLV-I/II infections in Brazil, were assessed within an ongoing cooperative research. OBJECTIVE: The study assessed the seroprevalences of HIV-1 and HTLV-I/II infections, as well as the prevalence of HIV-1 subtypes in a sample of IDUs from Rio de Janeiro, Brazil. An attempt to evaluate HIV incidence was carried out using a dual 'sensitive/less sensitive' testing strategy. STUDY DESIGN: Cross-sectional evaluation of 175 IDUs. Serostatus for HIV-1 and HTLV-I/II were established by enzyme-linked immunosorbent assays, and confirmed by western blot. The dual testing strategy aimed to estimate HIV-1 incidence rates. Differentiation between HTLV-I and -II was performed by western blot. DNA samples were polymerase chain reaction amplified by a nested protocol, and HIV-1 subtyping was determined by heteroduplex mobility assay. RESULTS: Forty-six and 29 samples were found to be, respectively, positive for HIV-1 and HTLV-I/II, 15 of them co-infected by both viruses. Among HTLV-I/II-infected patients, 75.9% were infected by HTLV-I. Thirty-one HIV samples were identified as B subtype, with seven of them showing the typical "Brazilian B" pattern in the gp120 V3 loop, and ten were identified as F subtype. The use of less sensitive assays for HIV infection wrongly identified a deeply immunocompromised patient as an incident case. CONCLUSION: Moderately high seroprevalences were found for both HIV-1 and HTLV-I/II infections, HIV-1/HTLV-I co-infections being of special concern. A non-statistically significant higher prevalence of F subtype was observed, when compared with the distribution of F/B subtypes among Brazilian patients from other exposure categories. No recent HIV-1 infections were detected, but a limitation of the "sensitive/less-sensitive" testing strategy was made evident.
dc.formatapplication/pdf
dc.languageeng
dc.publisherElsevier
dc.rightsopen access
dc.subjectInjecting drug users
dc.subjectHIV
dc.subjectHTLV
dc.subjectHIV/HTLV co-infection
dc.subjectSensitive/less sensitive dual testing strategy for HIV infection
dc.subjectInfecções por HIV/epidemiologia
dc.subjectHIV-1/classificação
dc.subjectInfecções por HTLV-I/epidemiologia
dc.subjectAbuso de Substâncias por Via Intravenosa/complicações
dc.subjectAdulto
dc.subjectBrasil/epidemiologia
dc.subjectEstudos Transversais
dc.subjectFeminino
dc.subjectInfecções por HIV/complicações
dc.subjectSoroprevalência de HIV
dc.subjectInfecções por HTLV-I/complicações
dc.subjectHumanos
dc.subjectMasculino
dc.subjectIncidência
dc.titleRetrovirus infections in a sample of injecting drug users in Rio de Janeiro City, Brazil: prevalence of HIV-1 subtypes, and co-infection with HTLV-I/II.
dc.typeArticle


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