Genotypic characterization and molecular evolution of avian reovirus in poultry flocks from Brazil

dc.creatorDe Carli, Silvia
dc.creatorWolf, Jonas Michel
dc.creatorGräf, Tiago
dc.creatorLehmann, Fernanda K. M.
dc.creatorFonseca, André S. K.
dc.creatorCanal, Cláudio W.
dc.creatorLungea, Vagner R.
dc.creatorIkuta, Nilo
dc.date2020-10-19T12:26:53Z
dc.date2020-10-19T12:26:53Z
dc.date2020
dc.date.accessioned2023-09-26T20:12:14Z
dc.date.available2023-09-26T20:12:14Z
dc.identifierDE CARLI, Silvia et al. Genotypic characterization and molecular evolution of avian reovirus in poultry flocks from Brazil. Avian Pathology, 2020.
dc.identifier0307-9457
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/44046
dc.identifier10.1080/03079457.2020.1804528
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8851265
dc.descriptionAvian reovirus (ARV) is one of the main causes of infectious arthritis/tenosynovitis and malabsorption syndrome (MAS) in poultry. ARVs have been disseminated in Brazilian poultry flocks in the last years. This study aimed to genotype ARVs and to evaluate the molecular evolution of the more frequent ARV lineages detected in Brazilian poultry-producing farms. A total of 100 poultry flocks with clinical signs of tenosynovitis/MAS, from all Brazilian poultryproducing regions were positive for ARV by PCR. Seventeen bird tissues were submitted to cell culture and ARV RNA detection/genotyping by two PCRs. The phylogenetic classification was based on σC gene alignment using a dataset with other Brazilian and worldwide ARVs sequences. ARVs were specifically detected by both PCRs from the 17 cell cultures, and σC gene partial fragments were sequenced. All these sequences were aligned with a total of 451 ARV σC gene data available in GenBank. Phylogenetic analysis demonstrated five welldefined clusters that were classified into lineages I, II, III, IV, and V. Three lineages could be further divided into sub-lineages: I (I vaccine, Ia, Ib), II (IIa, IIb, IIc) and IV (IVa and IVb). Brazilian ARVs were from four lineages/sub-lineages: Ib (48.2%), IIb (22.2%), III (3.7%) and V (25.9%). The Bayesian analysis demonstrated that the most frequent sub-lineage Ib emerged in the world around 1968 and it was introduced into Brazil in 2010, with increasing spread soon after. In conclusion, four different ARV lineages are circulating in Brazilian poultry flocks, all associated with clinical diseases.
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) [311010/ 2017-2], Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) [Finance Code 001], Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul (FAPERGS) and Pro Reitoria de Pesquisa Universidade Federal do Rio Grande do Sul.
dc.formatapplication/pdf
dc.languageeng
dc.publisherTaylor & Francis
dc.rightsopen access
dc.subjectARV
dc.subjectTenossinovite
dc.subjectArtrite
dc.subjectFilogenia
dc.subjectEvolução viral
dc.subjectARV
dc.subjectTenosynovitis
dc.subjectArthritis
dc.subjectMalabsorption syndrome
dc.subjectPhylogeny
dc.subjectViral evolution
dc.titleGenotypic characterization and molecular evolution of avian reovirus in poultry flocks from Brazil
dc.typeArticle


Este ítem pertenece a la siguiente institución