Characterization of novel Leishmania infantum recombinant proteins encoded by genes from five families with distinct capacities for serodiagnosis of canine and human visceral leishmaniasis

dc.creatorOliveira, Geraldo Gileno de Sá
dc.creatorMagalhães, Franklin Barbalho
dc.creatorTeixeira, Márcia Cristina Aquino
dc.creatorPereira, Andréa Mendes
dc.creatorPinheiro, Cristiane Garboggini Melo de
dc.creatorSantos, Lenita Ramires dos
dc.creatorNascimento, Marília B
dc.creatorBedor, Cheila Nataly Galindo
dc.creatorAlbuquerque, Alessandra B. Lima
dc.creatordosSantos, Washington Luis Conrado
dc.creatorGomes, Yara de Miranda
dc.creatorMoreira Júnior, Edson Duarte
dc.creatorBrito, Maria E. F. de
dc.creatorPontes-de-Carvalho, Lain Carlos
dc.creatorMelo Neto, Osvaldo Pompílio de
dc.date2014-02-07T14:25:50Z
dc.date2014-02-07T14:25:50Z
dc.date2011
dc.date.accessioned2023-09-26T20:11:18Z
dc.date.available2023-09-26T20:11:18Z
dc.identifierOLIVEIRA, G. G. et al. Characterization of novel Leishmania infantum recombinant proteins encoded by genes from five families with distinct capacities for serodiagnosis of canine and human visceral leishmaniasis. American Journal of Tropical Medicine and Hygiene, v. 85, n. 6, p. 1025-1034, 2011.
dc.identifier1476-1645
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/7296
dc.identifier10.4269/ajtmh.2011.11-0102
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8850828
dc.descriptionTo expand the available panel of recombinant proteins that can be useful for identifying Leishmania -infected dogs and for diagnosing human visceral leishmaniasis (VL), we selected recombinant antigens from L. infantum , cDNA, and genomic libraries by using pools of serum samples from infected dogs and humans. The selected DNA fragments encoded homologs of a cytoplasmic heat-shock protein 70, a kinesin, a polyubiquitin, and two novel hypothetical proteins. Histidine-tagged recombinant proteins were produced after subcloning these DNA fragments and evaluated by using an enzyme-linked immunosorbent assays with panels of canine and human serum samples. The enzyme-linked immunosorbent assays with different recombinant proteins had different sensitivities (67.4–93.0% and 36.4–97.2%) and specificities (76.1–100% and 90.4–97.3%) when tested with serum samples from Leishmania -infected dogs and human patients with VL. Overall, no single recombinant antigen was sufficient to serodiagnosis all canine or human VL cases.
dc.formatapplication/pdf
dc.languageeng
dc.publisherThe American Society of Tropical Medicine and Hygiene
dc.rightsopen access
dc.subjectDoenças do Cão/diagnóstico
dc.subjectGenes de Protozoários/genética
dc.subjectLeishmania infantum/genética
dc.subjectLeishmaniose Visceral/diagnóstico
dc.subjectAnimais
dc.subjectAntígenos de Protozoários/genética
dc.subjectClonagem Molecular
dc.subjectDoenças do Cão/parasitologia
dc.subjectEnsaio de Imunoadsorção Enzimática/métodos
dc.subjectEnsaio de Imunoadsorção Enzimática/veterinária
dc.subjectHumanos
dc.subjectProteínas de Choque Térmico HSP70/genética
dc.subjectCinesina/genética
dc.subjectLeishmaniose Visceral/parasitologia
dc.subjectLeishmaniose Visceral/veterinária
dc.subjectPoliubiquitina/genética
dc.subjectProteínas Recombinantes/genética
dc.subjectSensibilidade e Especificidade
dc.subjectTestes Sorológicos/métodos
dc.subjectTestes Sorológicos/veterinária
dc.titleCharacterization of novel Leishmania infantum recombinant proteins encoded by genes from five families with distinct capacities for serodiagnosis of canine and human visceral leishmaniasis
dc.typeArticle


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