Lipids From Trypanosoma cruzi Amastigotes of RA and K98 Strains Generate a Pro-inflammatory Response via TLR2/6

dc.creatorBott, Emanuel
dc.creatorCarneiro, Alan B.
dc.creatorGimenez, Guadalupe
dc.creatorLópez, María G.
dc.creatorLammel, Estela M.
dc.creatorAtella, Georgia C.
dc.creatorBozza, Patrícia T.
dc.creatorBelaunzarán, María L.
dc.date2018-10-04T11:20:29Z
dc.date2018-10-04T11:20:29Z
dc.date2018
dc.date.accessioned2023-09-26T20:10:55Z
dc.date.available2023-09-26T20:10:55Z
dc.identifierBOTT, Emanuel; et al. Lipids From Trypanosoma cruzi Amastigotes of RA and K98 Strains Generate a Pro-inflammatory Response via TLR2/6. Frontiers in Celluar and Infection Microbiology, v.8, Article 151, 14p, May 2018.
dc.identifier2235-2988
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/29312
dc.identifier10.3389/fcimb.2018.00151
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8850641
dc.descriptionLipids from microorganisms are ligands of Toll like receptors (TLRs) and modulate the innate immune response. Herein, we analyze in vitro the effect of total lipid extracts from Trypanosoma cruzi amastigotes of RA and K98 strains (with polar biological behavior) on the induction of the inflammatory response and the involvement of TLRs in this process. We demonstrated that total lipid extracts from both strains induced lipid body formation, cyclooxygenase-2 expression and TNF-α and nitric oxide release in macrophages, as well as NF-κB activation and IL-8 release in HEK cells specifically through a TLR2/6 dependent pathway. We also evaluated the inflammatory response induced by total lipid extracts obtained from lysed parasites that were overnight incubated to allow the action of parasite hydrolytic enzymes, such as Phospholipase A1, over endogenous phospholipids. After incubation, these total lipid extracts showed a significantly reduced pro-inflammatory response, which could be attributed to the changes in the content of known bioactive lipid molecules like lysophospholipids and fatty acids, here reported. Moreover, analyses of total fatty acids in each lipid extract were performed by gas chromatography-mass spectrometry. Our results indicate a relevant role of T. cruzi lipids in the induction of a pro-inflammatory response through the TLR2/6 pathway that could contribute to the modulation of the immune response and host survival.
dc.formatapplication/pdf
dc.languageeng
dc.publisherFrontiers Media
dc.rightsopen access
dc.subjectTrypanosoma cruzi
dc.subjectLipídeos
dc.subjectpró-inflamatório
dc.subjectFosfolipases A1
dc.subjectTrypanosoma cruzi
dc.subjectlipids
dc.subjectpro-inflammatory
dc.subjectphospholipase A1
dc.subjectTLR2/6
dc.titleLipids From Trypanosoma cruzi Amastigotes of RA and K98 Strains Generate a Pro-inflammatory Response via TLR2/6
dc.typeArticle


Este ítem pertenece a la siguiente institución