Genetic characterization of morphologically variant strains of Paracoccidioides brasiliensis

dc.creatorBorba, Cintia de Moraes
dc.creatorCorreia, Janaina
dc.creatorVinhas, Ester
dc.creatorMartins, Albert
dc.creatorAlves, Beatriz Costa Aguiar
dc.creatorUnkles, Sheila
dc.creatorKinghorn, James Robertson
dc.creatorSilva, Norma Lucena
dc.date2018-08-14T14:06:11Z
dc.date2018-08-14T14:06:11Z
dc.date2008
dc.date.accessioned2023-09-26T20:10:45Z
dc.date.available2023-09-26T20:10:45Z
dc.identifierBORBA, Cintia de Moraes; et al. Genetic characterization of morphologically variant strains of Paracoccidioides brasiliensis. Mem Inst Oswaldo Cruz, v.103, n.3, p.306-309, May 2008.
dc.identifier0074-0276
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/28129
dc.identifier1678-8060
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8850564
dc.descriptionMolecular characterization of Paracoccidioides brasiliensis variant strains that had been preserved under mineral oil for decades was carried out by random amplified polymorphic DNA analysis (RAPD). On P. brasiliensis variants in the transitional phase and strains with typical morphology, RAPD produced reproducible polymorphic amplification products that differentiated them. A dendrogram based on the generated RAPD patterns placed the 14 P. brasiliensis strains into five groups with similarity coefficients of 72%. A high correlation between the genotypic and phenotypic characteristics of the strains was observed. A 750 bp-RAPD fragment found only in the wild-type phenotype strains was cloned and sequenced. Genetic similarity analysis using BLASTx suggested that this RAPD marker represents a putative domain of a hypothetical flavin-binding monooxygenase (FMO)-like protein of Neurospora crassa.
dc.formatapplication/pdf
dc.languageeng
dc.publisherFundação Oswaldo Cruz. Instituto Oswaldo Cruz
dc.rightsopen access
dc.subjectPararacoccidiodes brasiliensis
dc.subjectcepas
dc.subjectCaracterização genética
dc.subjectFMO
dc.subjectPararacoccidiodes brasiliensis
dc.subjectRAPD-PCR
dc.subjectGenetic characterization
dc.subjectTécnica de Amplificação ao Acaso de DNA Polimórfico
dc.titleGenetic characterization of morphologically variant strains of Paracoccidioides brasiliensis
dc.typeArticle


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