Immunome and venome of Bothrops jararacussu: a proteomic approach to study the molecular immunology of snake toxins

dc.creatorCorrea Netto, Carlos
dc.creatorAraujo, Ricardo Teixeira
dc.creatorAguiar, Aniesse Silva
dc.creatorMelgarejo, Aníbal Rafael
dc.creatorDe Simone, Salvatore Giovanni
dc.creatorSoares, Márcia Regina
dc.creatorFoguel, Debora
dc.creatorZingali, Russolina Benedeta
dc.date2017-09-19T15:37:34Z
dc.date2017-09-19T15:37:34Z
dc.date2010
dc.date.accessioned2023-09-26T20:10:23Z
dc.date.available2023-09-26T20:10:23Z
dc.identifierCORREA NETO, Carlos; et al. Immunome and venome of Bothrops jararacussu: A proteomic approach to study the molecular immunology of snake toxins. Toxicon, v.55, p.1222-1235, 2010.
dc.identifier0041-0101
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/21060
dc.identifier10.1016/j.toxicon.2009.12.018
dc.identifier1879-3150
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8850396
dc.descriptionA combination of anti-bothropic and anti-crotalic sera has been reported to be more effective in neutralizing the effects of Bothrops jararacussu venom than anti-bothropic serum alone. The role of proteins from B. jararacussu venom in the horse immune response was evaluated via the analysis of cross-reactivity with homologous and heterologous sera. Many of the proteins in B. jararacussu venom were identified via 2D gel electrophoresis. Western blots revealed that anti-jararacussu showed higher reactivity to l-aminoxidase (LAOs) and snake venom metalloproteinase, (SVMPs) and weaker reactivity towards Snake venom serine proteases (SVSPs), PLA(2), C-type lectin and cysteine-rich proteins. Anti-jararaca preferentially recognized LAOs, SVMPs and SVSPs. Both of these sera failed to recognize low-molecular weight proteins. Anti-crotalic serum clearly recognized LAOs, C-type lectin, SVSP, cysteine-rich proteins, SVMP and Asp49-PLA(2). The cross-reactivity with anti-PLA(2) revealed the immunoreactivity of these antibodies to proteins with molecular masses in a range that is poorly recognized by other studied anti-sera. Our results suggest that the contribution of anti-crotalic serum to the neutralization of B. jararacussu by may be due to its cross-reactivity with proteins such as C-type lectins, SVSPs, Asp49-PLA(2). These results also reinforce the importance of neutralizing the highly toxic proteins inclusive those with low immunogenicity in commercial antivenom production to obtain a highly protective serum against snake venoms.
dc.description2030-01-01
dc.formatapplication/pdf
dc.languageeng
dc.publisherElsevier
dc.rightsrestricted access
dc.subjectBothrops
dc.subjectVenenos de Serpentes
dc.subjectBothrops jararacussu
dc.subjectBothrops jararaca
dc.subjectCrotalus durissus
dc.subjectSnake venom proteome
dc.subjectImmunome
dc.titleImmunome and venome of Bothrops jararacussu: a proteomic approach to study the molecular immunology of snake toxins
dc.typeArticle


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