STAT3 gene expression in ameloblastomas and odontogenic keratocysts

Експресија гена STAT3 код амелобластома и одонтогених кератоциста

dc.creatorMelo, Leonardo de Araújo
dc.creatorSantana, Dandara Andrade de
dc.creatorSales, Caroline Brandi Schlaepfer
dc.creatorCarneiro Júnior, Bráulio
dc.creatorMartins, Marília Trierveiler
dc.creatorFreitas, Valéria Souza
dc.creatorXavier, Flávia Caló Aquino
dc.creatorCury, Patrícia Ramos
dc.creatorGurgel, Clarissa Araújo Silva
dc.creatorSantos, Jean Nunes dos
dc.date2023-02-13T18:37:10Z
dc.date2023-02-13T18:37:10Z
dc.date2022
dc.date.accessioned2023-09-26T20:10:23Z
dc.date.available2023-09-26T20:10:23Z
dc.identifierMELO, Leonardo de Araújo et al. STAT3 gene expression in ameloblastomas and odontogenic keratocyst. Srpski Arhiv za Celokupno Lekarstvo, p. 1-16, 2022.
dc.identifier2406-0895
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/57050
dc.identifier10.2298/SARH220607115A
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8850389
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico Desenvolvimento (CNPq). Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES).
dc.descriptionIntroduction/Objective. STAT3 (signal transducers and activators of transcription) is involved in different physiological processes, including cell proliferation and survival. High expression of this protein is observed in various types of cancer. This study aimed to investigate the gene and protein expression of STAT3 in a series of odontogenic cysts and tumors to provide more information about their biological profile. Methods. The STAT3 gene expression at mRNA was quantified by real-time quantitative polymerase chain reaction (RT-qPCR) in 23 odontogenic keratocysts (OKCs) and 7 ameloblastomas (AMs), and compared to the non-neoplastic oral mucosa. We also assessed the expression of STAT3 gene at protein levels, using immunohistochemistry, in 43 OKCs and 47 AMs. Results. STAT3 transcripts were found in 96.6% of the tumors studied; however, the gene was downregulated in OKC and AM compared to the non-neoplastic oral mucosa. The STAT3 gene expression at mRNA level was higher in sporadic OKC than syndromic OKC (p = 0.04). There was no difference in STAT3 gene expression at mRNA level between OKCs and AMs (p = 0.88). Immunostaining of STAT3 revealed no significant difference between sporadic and syndrome OKC (p > 0.05), nor between conventional and unicystic AMs (p > 0.05). Ameloblastomas exhibited significantly higher STAT3 immunostaining than OKCs (p = 0.03). In OKC and AM, STAT3 immunostaining was predominantly cytoplasmic and no difference in the cellular localization of STAT3 was observed between these lesions (p = 0.58). Conclusion. Our findings showed low expression of STAT3 gene in OKCs and AMs in relation to non-neoplastic oral mucosa. However, higher STAT3 immunostaining was observed in AMs compared to OKCs.
dc.formatapplication/pdf
dc.languageeng
dc.publisherSrpski Lekarski Drustvo
dc.rightsopen access
dc.subjectCistos odontogênicos
dc.subjectAmeloblastoma
dc.subjectFator de transcrição STAT3
dc.subjectExpressão genética
dc.subjectImuno-histoquímica
dc.subjectOdontogenic cysts
dc.subjectAmeloblastoma
dc.subjectSTAT3 transcription factor
dc.subjectGene expression
dc.subjectImmunohistochemistry
dc.subjectCistos odontogênicos
dc.subjectAmeloblastoma
dc.subjectFator de Transcrição STAT3
dc.subjectGenética
dc.subjectImuno-histoquímica
dc.titleSTAT3 gene expression in ameloblastomas and odontogenic keratocysts
dc.titleЕкспресија гена STAT3 код амелобластома и одонтогених кератоциста
dc.typeArticle


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