Lignins isolated from Prickly pear cladodes of the species Opuntia fícus-indica (Linnaeus) Miller and Opuntia cochenillifera (Linnaeus) Miller induces mice splenocytes activation, proliferation and cytokines production

dc.creatorCruz Filho, Iranildo José da
dc.creatorBarros, Bárbara Rafaela da Silva
dc.creatorAguiar, Lethícia Maria de Souza
dc.creatorNavarro, Claudia Daniele Carvalho
dc.creatorRuas, Juliana Silveira
dc.creatorLorena, Virgínia Maria Barros de
dc.creatorRocha, George Jackson de Moraes
dc.creatorVercesi, Aníbal Eugênio
dc.creatorMelo, Cristiane Moutinho Lagos de
dc.creatorMaior, Ana Maria Souto
dc.date2019-10-16T14:11:34Z
dc.date2019-10-16T14:11:34Z
dc.date2019
dc.date.accessioned2023-09-26T20:10:17Z
dc.date.available2023-09-26T20:10:17Z
dc.identifierCRUZ FILHO, Iranildo José da et al. Lignins isolated from Prickly pear cladodes of the species Opuntia fícus-indica (Linnaeus) Miller and Opuntia cochenillifera (Linnaeus) Miller induces mice splenocytes activation, proliferation and cytokines production. International Journal of Biological Macromolecules, v. 123, p. 1331-1339, Feb. 2019.
dc.identifier0141-8130
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/36491
dc.identifier10.1016/j.ijbiomac.2018.09.120
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8850334
dc.descriptionOpuntia fícus-indica and Opuntia cochenillifera are species of Cactaceae, found in the arid regions of the planet. They present water, cellulose, hemicellulose, pectins, extractives, ashes and lignins. Here we aimed to study the immunomodulatory action of lignins from these two species against mice splenocytes, since no study for this purpose has yet been reported. The antioxidant activities of these lignins were evaluated by the DPPH, ABTS, NO assays and total antioxidant activity. Cytotoxicity was evaluated through Annexin V-FITC and propidium iodide-PE probs and cell proliferation was determined by CFSE. Immunomodulation studies with Opuntia lignins obtained were performed through investigation of ROS levels, cytosolic calcium release, changes on mitochondrial membrane potential, cytokine production and NO release. Results showed that Opuntia cochenillifera lignin presented more phenolic amount and antioxidant activities than Opuntia ficius-indica. Both lignins showed high cell viability (>96%) and cell proliferation. Activation signal was observed for both lignins with increase of ROS and cytosolic calcium levels, and changes in mitochondrial membrane potential. In addition, lignins induced high TNF-α, IL-6 and IL-10 production and reduced NO release. Therefore, these lignins present great potential to be used as molecules with a proinflammatory profile, being shown as a promising therapeutic agent.
dc.description2050-01-01
dc.formatapplication/pdf
dc.languageeng
dc.rightsrestricted access
dc.subjectCytokines
dc.subjectImmunomodulation
dc.subjectLignins
dc.subjectMacromolecules
dc.subjectAnimais
dc.subjectAntioxidantes / farmacologia
dc.subjectCálcio / metabolismo
dc.subjectEspectroscopia de Ressonância Magnética Nuclear de Carbono-13
dc.subjectMorte Celular / efeitos dos fármacos
dc.subjectProliferação de Células / efeitos dos fármacos
dc.subjectCitocinas / biossíntese
dc.subjectCitosol / metabolismo
dc.subjectFeminino
dc.subjectLignina / isolamento & purificação
dc.subjectLignina / farmacologia
dc.subjectPotencial da Membrana Mitocondrial / efeitos dos fármacos
dc.subjectCamundongos Endogâmicos BALB C
dc.subjectÓxido Nítrico / metabolismo
dc.subjectOpuntia / química
dc.subjectEstresse Oxidativo / efeitos dos fármacos
dc.subjectFenóis / análise
dc.subjectEspécies Reativas de Oxigênio / metabolismo
dc.subjectBaço / citologia
dc.titleLignins isolated from Prickly pear cladodes of the species Opuntia fícus-indica (Linnaeus) Miller and Opuntia cochenillifera (Linnaeus) Miller induces mice splenocytes activation, proliferation and cytokines production
dc.typeArticle


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