Detection of norovirus epidemic genotypes in raw sewage using next generation sequencing

dc.creatorFumian, Tulio Machado
dc.creatorFioretti, Julia M.
dc.creatorLun, Jennifer H.
dc.creatorSantos, Ingrid A. L. dos
dc.creatorWhite, Peter A.
dc.creatorMiagostovich, Marize P.
dc.date2019-05-02T12:54:52Z
dc.date2019-05-02T12:54:52Z
dc.date2019
dc.date.accessioned2023-09-26T20:09:40Z
dc.date.available2023-09-26T20:09:40Z
dc.identifierFUMIAN, Tulio M. et al. Detection of norovirus epidemic genotypes in raw sewage using next generation sequencing. Environmental International, v. 123, p.282-291, 2019.
dc.identifier0160-4120
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/32905
dc.identifier10.1016/j.envint.2018.11.054
dc.identifier1873-6750
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8850074
dc.descriptionNoroviruses are a leading cause of epidemic and pandemic acute gastroenteritis (AGE) worldwide, and contaminated food and water are important routes for its transmission. Raw sewage has been used for viral surveillance to monitor the emergence of new norovirus strains with the potential to cause epidemics. In this study, we investigated norovirus occurrence and norovirus RNA levels in 156 samples collected from May 2013 to May 2014, across three different stages (52 samples each) of a wastewater treatment plant (WWTP) in Rio de Janeiro, Brazil. We also explored norovirus GII diversity in raw sewage samples by next-sequencing generation (NGS). In addition, we examined norovirus prevalence and molecular epidemiology from acute gastroenteritis cases. Using RT-qPCR, norovirus GI and GII was detected in 38.5% and 96.1% of raw sewage samples, 40.4% and 96.1% of primary effluent samples and 1.9% and 5.8% of final effluent samples, respectively. Norovirus RNA levels varied from 4 to 6.2 log10 genome copies per litre (gc L-1) for GI and from 4.4 to 7.3 log10 gc L-1 for GII. Using MiSeq NGS, we identified 13 norovirus genotypes over the one-year period, with six dominant capsid genotypes, including GII.4, GII.17, GII.5, GII.2, GII.3 and GII.1. GII.4 noroviruses were the most prevalent in wastewater samples (68.5%), and a similar trend was observed in AGE cases (71%). The emergent GII.17 was the second most prevalent genotype (14.3%) identified in the raw sewage samples, however, it was not detected in clinical cases. Due to the high burden of norovirus outbreaks and the lack of vaccine and antiviral drugs, it is essential to understand the genotypic diversity of norovirus at the population level. Complementary data obtained from both clinical and environmental (sewage) samples proved to be an effective strategy to monitor the circulation and emergence of norovirus epidemic genotypes.
dc.formatapplication/pdf
dc.languageeng
dc.publisherElsevier
dc.rightsopen access
dc.subjectNorovirus
dc.subjectÁguas residuais
dc.subjectVigilância Ambiental
dc.subjectSequenciamento de próxima geração
dc.subjectAnálise filogenética
dc.subjectNoroviruses
dc.subjectWastewater
dc.subjectEnvironmental surveillance
dc.subjectNext-generation sequencing
dc.subjectPhylogenetic analysis
dc.titleDetection of norovirus epidemic genotypes in raw sewage using next generation sequencing
dc.typeArticle


Este ítem pertenece a la siguiente institución