TNF-alpha is expressed at sites of parasite and tissue destruction in the spleen of mice acutely infected with Trypanosoma cruzi.

dc.creatorLima, Elianita Suzart de
dc.creatorAndrade, Zilton de Araújo
dc.creatorAndrade, Sonia Gumes
dc.date2012-09-10T21:09:10Z
dc.date2012-09-10T21:09:10Z
dc.date2001
dc.date.accessioned2023-09-26T20:08:54Z
dc.date.available2023-09-26T20:08:54Z
dc.identifierLIMA, E.S.; ANDRADE, Z.A.; ANDRADE, S.G. TNF-alpha is expressed at sites of parasite and tissue destruction in the spleen of mice acutely infected with Trypanosoma cruzi. International Journal of Experimental Pathology, v. 82, n. 6, p. 327-36, Dec. 2001.
dc.identifier0959-9673
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/5500
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8849713
dc.descriptionMice infected with a macrophagotropic strain of Trypanosoma cruzi develop progressive splenomegaly due to reactive hyperplasia with increased number of lymphocytes and macrophages, culminating in parasite disintegration and necrosis of parasitized cells. Necrotic changes have been attributed to the liberation of toxic cytokines, including TNF-alpha, from parasitized macrophages. In the present study, the presence of TNF-alpha was investigated in situ. In addition the participation of destroyed parasites in inducing the liberation of TNF-alpha was examined in two highly susceptible mice strains (C3H and Swiss) and a more resistant strain (DBA). Swiss (90) C3H/He (83) and DBA (30) mice were infected with the Peruvian strain of T. cruzi. Nineteen infected Swiss mice, and 22 infected C3H/He were treated with Benznidazole (one or two doses, 100 mg/kg bw/day), on the 8th and 9th days after infection. Necrotic splenic lesions occurred in both susceptible and resistant strains of mice. Although differing in degree, lesions were more intense in C3H and Swiss than in DBA mice. Comparing untreated and treated susceptible mice, necrotic lesions were significantly less intense in the latter. By specific monoclonal antibody immunolabelling, TNF-alpha was demonstrated in the cytoplasm of macrophages and within necrotic areas, from Swiss, C3H/He and DBA mouse spleens. In conclusion, TNF-alpha, probably synthesized by macrophages, was strongly expressed at the sites of parasite and cell destruction, thus appearing to play a pivotal role in splenic necrotic changes associated with severe experimental T. cruzi infection.
dc.formatapplication/pdf
dc.languageeng
dc.rightsopen access
dc.subjectTrypanosoma cruzi
dc.subjectSpleen necrosis
dc.subjectMacrophage parasitism
dc.subjectTNF-a
dc.subjectC3H/He mice
dc.subjectDBA mice
dc.subjectBenznidazole
dc.subjectDoença de Chagas/metabolismo
dc.subjectBaço/metabolismo
dc.subjectFator de Necrose Tumoral alfa/metabolismo
dc.subjectDoença Aguda
dc.subjectDoença de Chagas/quimioterapia
dc.subjectDoença de Chagas/patologia
dc.subjectSuscetibilidade a Doenças
dc.subjectTécnicas Imunoenzimáticas
dc.subjectMacrófagos/metabolismo
dc.subjectCamundongos Endogâmicos C3H
dc.subjectCamundongos Endogâmicos DBA
dc.subjectNecrose
dc.subjectNitroimidazóis/uso terapêutico
dc.subjectParasitemia/metabolismo
dc.subjectBaço/patologia
dc.subjectTaxa de Sobrevida
dc.subjectTripanossomicidas/uso terapêutico
dc.titleTNF-alpha is expressed at sites of parasite and tissue destruction in the spleen of mice acutely infected with Trypanosoma cruzi.
dc.typeArticle


Este ítem pertenece a la siguiente institución