Genetic differences between two Leishmania major-like strains revealed by suppression subtractive hybridization

dc.creatorWu, Ângela Cardoso de Alvarenga
dc.creatorFreitas, Michelle Aparecida Ribeiro de
dc.creatorSilva, Soraia de O
dc.creatorNogueira, Paula Monalisa
dc.creatorSoares, Rodrigo Pedro Pinto
dc.creatorPesquero, João Bosco
dc.creatorGomes, Maria Aparecida
dc.creatorPesquero, Jorge Luiz
dc.creatorMelo, Maria Norma
dc.date2016-04-01T18:16:53Z
dc.date2016-04-01T18:16:53Z
dc.date2015
dc.date.accessioned2023-09-26T20:08:35Z
dc.date.available2023-09-26T20:08:35Z
dc.identifierWU, Ângela Cardoso de Alvarenga et al. Genetic differences between two Leishmania major-like strains revealed by suppression subtractive hybridization. Molecular and Biochemical Parasitology, Vol. 203, n. 1–2, p. 34–38, 2015
dc.identifier0166-6851
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/13439
dc.identifier10.1016/j.molbiopara.2015.10.005
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8849575
dc.descriptionLeishmania major, the causative agent of zoonotic leishmaniasis, is restricted to Old World countries. Molecular and biochemical techniques have been used to identify some L. major-like isolated in South America including Brazil. Here, two L. major-like strains, one virulent (BH49) and one non-virulent (BH121), were subjected to suppression subtractive hybridization (SSH) technique in order to identify differentially expressed genes. SSH technique identified nine cDNA fragments exhibiting high homology to previously sequenced L. major genes. Five cDNAs (four specific for BH49 and one for BH121) were confirmed by RT-PCR. Among those differentially expressed subtracted genes, some were involved in physiological processes including metabolism, translation and destination of proteins, production of energy, virulence factors and unknown functions. Western-blot analysis confirmed a higher expression level of β-1,3-galactosyl residues in L. major-like lipophosphoglycan (LPG). This molecular analysis opens the possibility for identification of potential virulence factors not only in different strains, but also in others species of Leishmania.
dc.formatapplication/pdf
dc.languageeng
dc.publisherElsevier Ltd
dc.rightsrestricted access
dc.subjectLeishmania major-like
dc.subjectVirulence
dc.subjectDifferential gene expression
dc.subjectLipophosphoglycan
dc.subjectPhosphoglycan β-1,3-galactosyltransferase
dc.titleGenetic differences between two Leishmania major-like strains revealed by suppression subtractive hybridization
dc.typeArticle


Este ítem pertenece a la siguiente institución