Distribution of latent bovine herpesvirus 2 DNA in tissues of experimentally infected sheep

dc.creatorTorres, F. D.
dc.creatorAlmeida, S. R.
dc.creatorSilva, M. S.
dc.creatorWeiblen, R.
dc.creatorFlores, E. F.
dc.date2018-08-06T12:02:21Z
dc.date2018-08-06T12:02:21Z
dc.date2009
dc.date.accessioned2023-09-26T20:08:15Z
dc.date.available2023-09-26T20:08:15Z
dc.identifierTORRES, F. D. et al. Distribution of Latent Bovine Herpesvirus 2 DNA in Tissues of Experimentally Infected Sheep. Research in Veterinary Science, v. 87, n. 1, p. 161–166, ago. 2009.
dc.identifier1532-2661
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/27851
dc.identifier10.1016/j.rvsc.2008.12.003
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8849422
dc.descriptionA FD Torres possui assistências do CNPq. EF Flores (101666 / 2004-0) e R. Weiblen (301339 / 2004-0) são bolsistas de pesquisa do CNPq.
dc.descriptionThe biology of latent infection by bovine herpesvirus 2 (BoHV-2), the agent of mammillitis in cows, remains largely unknown. We herein report attempts to reactivate the latent infection and investigated the sites of BoHV-2 latency in experimentally infected sheep. Ewes inoculated with BoHV-2 in the udder's skin shed virus for up to five days, developed mammillitis and seroconverted. However, attempts to reactivate latent infection by dexamethasone administration at day 40 pi failed. Nevertheless, viral DNA--and not infectious virus--was detected by PCR in several nerve ganglia and/or regional lymph nodes (LNs) of all animals at day 40 post-reactivation. Likewise, lambs previously inoculated with BoHV-2 in the nose harbored latent viral DNA in trigeminal ganglia, tonsils and regional LNs. These results demonstrate that BoHV-2 establishes latent infection in nerve ganglia and in regional lymphoid tissues, yet virus reactivation is not easily achieved by standard protocols used.
dc.description2050-01-01
dc.formatapplication/pdf
dc.languageeng
dc.rightsrestricted access
dc.subjectHerpesvirus bovino 2
dc.subjectBoHV-2
dc.subjectInfecção latente
dc.subjectOvelhas
dc.subjectBovine herpesvirus 2
dc.subjectBoHV-2
dc.subjectLatent infection
dc.subjectSheep
dc.subjectAnimais
dc.subjectDNA Viral / isolamento e purificação
dc.subjectDNA Viral / metabolismo
dc.subjectFêmea
dc.subjectHerpes simplex / veterinária
dc.subjectHerpes Simplex / virologia
dc.subjectHerpesvirus 2, bovino / genética
dc.subjectLactação
dc.subjectOvelhas
dc.subjectDoenças dos Ovinos / virologia
dc.subjectDistribuição de Tecidos
dc.subjectLatência do vírus
dc.subjectDerramamento de vírus
dc.titleDistribution of latent bovine herpesvirus 2 DNA in tissues of experimentally infected sheep
dc.typeArticle


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