Trypanosoma cruzi: ubiquity expression of surface cruzipain molecules in TCI and TCII field isolates

dc.creatorFampa, Patrícia
dc.creatorSantos, André L. S.
dc.creatorRamirez, Marcel I.
dc.date2017-09-12T16:12:35Z
dc.date2017-09-12T16:12:35Z
dc.date2010
dc.date.accessioned2023-09-26T20:08:12Z
dc.date.available2023-09-26T20:08:12Z
dc.identifierFAMPA, Patricia; SANTOS, André L. S.; RAMIREZ, Marcel I.Trypanosoma cruzi: ubiquity expression of surface cruzipain molecules in TCI and TCII field isolates. Parasitol Res, v.107, p.443–447, 2010.
dc.identifier0932-0113
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/20972
dc.identifier10.1007/s00436-010-1888-9
dc.identifier1432-1955
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8849401
dc.descriptionTrypanosoma cruzi presents considerable genetic and protein profile polymorphism among different parasite populations. In a previous work, our group indicated cysteine and metalloprotease activities as good markers for separating T. cruzi I (TCI) from T. cruzi II (TCII) isolates, with higher heterogeneity among TCII isolates. Here, we have investigated the expression level of surface cruzipain in 16 field isolates belonging to the genotype TCI (n = 8) and TCII (n = 8) of T. cruzi. By means of flow cytometry analyses, using an anti-cruzipain polyclonal antibody, we observed a highly heterogeneous pattern of surface cruzipain molecules in these isolates, independently of their genotypes, cell measurements (size and granularity), original hosts, or biomes. However, fluorescence labeling tended to be stronger in TCI than in TCII population. Interestingly, isolates that expressed higher levels of surface cruzipain also yielded elevated levels of metacyclogenesis in vitro.
dc.description2030-01-01
dc.formatapplication/pdf
dc.languageeng
dc.publisherSpringer Verlag (Germany)
dc.rightsrestricted access
dc.subjectTrypanosoma cruzi
dc.subjectTCI
dc.subjectatividades de cisteína
dc.subjectatividades de metaloprotease
dc.subjectMarcadores
dc.subjectTCII
dc.subjectTrypanosoma cruzi
dc.subjectFlow cytometry analyses
dc.subjectmetalloprotease activities
dc.subjectgood markers
dc.subjectcysteine activities
dc.titleTrypanosoma cruzi: ubiquity expression of surface cruzipain molecules in TCI and TCII field isolates
dc.typeArticle


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