Localization and function of Rhipicephalus (Boophilus) microplus vitellin-degrading cysteine endopeptidase

dc.creatorSeixas, Adriana
dc.creatorEstrela, Andréia B.
dc.creatorCeolato, Juliana C.
dc.creatorPontes, Emerson G.
dc.creatorLara, Flávio
dc.creatorGondim, Katia C.
dc.creatorTermignoni, Carlos
dc.date2018-04-26T13:35:45Z
dc.date2018-04-26T13:35:45Z
dc.date2010
dc.date.accessioned2023-09-26T20:08:02Z
dc.date.available2023-09-26T20:08:02Z
dc.identifierSEIXAS, Adriana; et al. Localization and function of Rhipicephalus (Boophilus) microplus vitellin-degrading cysteine endopeptidase. Parasitology, v.137, p.1819-1831, 2010.
dc.identifier0031-1820
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/26128
dc.identifier10.1017/S0031182010000624
dc.identifier1469-8161
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8849329
dc.descriptionThe tick Rhipicephalus (Boophilus) microplus is an important parasite of cattle in many areas of the tropics. Characterization of molecules involved in mechanisms such as vitellogenesis and embryo development may contribute to a better understanding of this parasite's physiology. The vitellin-degrading cysteine endopeptidase (VTDCE) is the most active enzyme involved in vitellin hydrolysis in R. microplus eggs. Here we show an association between VTDCE and vitellin in an additional site, apart from the active site. Our data also demonstrate cysteine endopeptidase activity in different tissues such as ovary, gut, fat body, salivary gland and female haemolymph, where it is controlled by a physiological inhibitor. In R. microplus female gut, VTDCE is localized in areas of protein synthesis and trafficking with the underlying haemolymph. VTDCE is also localized in the ovary basal region, in vesicle membranes of ovary pedicel cells and in oocyte cytosol. These results suggest that VTDCE plays a role in vitellin digestion during tick development.
dc.description2030-01-01
dc.formatapplication/pdf
dc.languageeng
dc.publisherCambridge University Press
dc.rightsrestricted access
dc.subjectRhipicephalus
dc.subjectVitelinas
dc.subjectInibidores
dc.subjectCisteína Endopeptidases
dc.subjectRhipicephalus (Boophilus) microplus
dc.subjectcysteine endopeptidase
dc.subjectcysteine endopeptidase inhibitor
dc.subjecthaemolymph
dc.subjectvitellin
dc.subjectVTDCE
dc.titleLocalization and function of Rhipicephalus (Boophilus) microplus vitellin-degrading cysteine endopeptidase
dc.typeArticle


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