Evaluation of parasitological examination, kDNA polymerase chain reaction and rK39-based immunochromatography for the diagnosis of visceral leishmaniasis in seropositive dogs from the screening-culling program in Brazil

dc.creatorSilva, Shara Regina da
dc.creatorDias, Consuelo Latorre Fortes
dc.creatorMichalsky, Érika Monteiro
dc.creatorSilva, João Carlos França
dc.creatorQuaresma, Patrícia Flávia
dc.creatorLima, Ana Cristina Vianna Mariano da Rocha
dc.creatorTeixeira Neto, Rafael Gonçalves
dc.creatorDias, Edelberto Santos
dc.date2015-03-26T18:52:44Z
dc.date2015-03-26T18:52:44Z
dc.date2014
dc.date.accessioned2023-09-26T20:07:59Z
dc.date.available2023-09-26T20:07:59Z
dc.identifierSILVA, Shara Regina et al. Evaluation of parasitological examination, kDNA polymerase chain reaction and rK39-based immunochromatography for the diagnosis of visceral leishmaniasis in seropositive dogs from the screening-culling program in Brazil. Revista da Sociedade Brasileira de Medicina Tropical, v. 47, n. 4, p. 462-468, 2014
dc.identifier0037-8682
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/9847
dc.identifier10.1590/0037-8682-0064-2014
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8849302
dc.descriptionIntroduction: Dogs play a primary role in the zoonotic cycle of visceral leishmaniasis (VL). Therefore, the accurate diagnosis of infected dogs, primarily asymptomatic dogs, is crucial to the efficiency of VL control programs. Methods: We investigated the agreement of four diagnostic tests for canine visceral leishmaniasis (CVL): parasite detection, either after myeloculture or by direct microscopic examination of tissue imprints; kinetoplast-deoxyribonucleic acid-polymerase chain reaction (kDNA-PCR); and an immunochromatographic test (ICT). An enzyme-linked immunosorbent assay (ELISA) and an indirect immunofluorescence test (IFAT), both of which were adopted as part of the screening-culling program in Brazil, were used as reference tests. Our sample set consisted of 44 seropositive dogs, 25 of which were clinically asymptomatic and 19 were symptomatic for CVL according to ELISA-IFAT. Results: The highest and lowest test co-positivities were observed for ICT (77.3%) and myeloculture (58.1%), respectively. When analyzed together, the overall percentage of co-positive tests was significantly higher for the symptomatic group compared to the asymptomatic group. However, only ICT was significantly different based on the results of a separate analysis per test for each group of dogs. The majority (93.8%) of animals exhibited at least one positive test result, with an average of 2.66 positive tests per dog. Half of the symptomatic dogs tested positive for all four tests administered. Conclusions: The variability between test results reinforces the need for more efficient and reliable methods to accurately diagnose canine VL, particularly in asymptomatic animals.
dc.formatapplication/pdf
dc.languageeng
dc.publisherSociedade Brasileira de Medicina Tropical
dc.rightsopen access
dc.subjectVisceral leishmaniasis
dc.subjectCanine visceral leishmaniasis
dc.subjectDiagnosis
dc.subjectLeishmania
dc.titleEvaluation of parasitological examination, kDNA polymerase chain reaction and rK39-based immunochromatography for the diagnosis of visceral leishmaniasis in seropositive dogs from the screening-culling program in Brazil
dc.typeArticle


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