dc.creatorTeixeira, Leonardo K.
dc.creatorFonseca, Bruna P. F.
dc.creatorAbreu, Adriana Vieira de
dc.creatorBarboza, Bianca A.
dc.creatorRobbs, Bruno K.
dc.creatorBozza, Patrícia T.
dc.creatorViola, João P. B.
dc.date2020-04-21T14:09:41Z
dc.date2020-04-21T14:09:41Z
dc.date2005
dc.date.accessioned2023-09-26T20:07:55Z
dc.date.available2023-09-26T20:07:55Z
dc.identifierTEIXEIRA, Leonardo K. et al. IFN- Production by CD8 T Cells Depends on NFAT1 Transcription Factor and Regulates Th Differentiation. Journal of Immunology, v. 175, p. 5931-5939, 2005.
dc.identifier0022-1767
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/40903
dc.identifier10.4049/jimmunol.175.9.5931
dc.identifier1550-6606
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8849273
dc.descriptionCD8+ T lymphocytes are excellent sources of IFN-gamma; however, the molecular mechanisms that dictate IFN-gamma expression upon TCR stimulation in these cells are not completely understood. In this study, we evaluated the involvement of NFAT1 in the regulation of IFN-gamma gene expression in murine CD8+ T cells and its relevance during Th differentiation. We show that CD8+, but not CD4+, T cells, represent the very first source of IFN-gamma upon primary T cell activation, and also that the IFN-gamma produced by naive CD8+ T cells may enhance CD4+ Th1 differentiation in vitro. TCR stimulation rapidly induced IFN-gamma expression in CD8+ T lymphocytes in a cyclosporin A-sensitive manner. Evaluation of CD8+ T cells showed that calcium influx alone was sufficient to activate NFAT1 protein, transactivate IFN-gamma gene promoter, and induce IFN-gamma production. In fact, NFAT1-deficient mice demonstrated highly impaired IFN-gamma production by naive CD8+ T lymphocytes, which were totally rescued after retroviral transduction with NFAT1-encoding vectors. Moreover, NFAT1-dependent IFN-gamma production by the CD8+ T cell compartment was crucial to control a Th2-related response in vivo, such as allergic inflammation. Consistently, CD8alpha- as well as IFN-gamma-deficient mice did not mount a Th1 immune response and also developed in vivo allergic inflammation. Our results clearly indicate that IFN-gamma production by CD8+ T cells is dependent of NFAT1 transcription factor and may be an essential regulator of Th immune responses in vivo.
dc.formatapplication/pdf
dc.languageeng
dc.publisherAmerican Association of Immunologists
dc.rightsopen access
dc.subjectCD8 T Cells
dc.subjectIFN-y
dc.subjectLInfócitos T
dc.subjectCD8 T Cells
dc.subjectIFN-y Production
dc.subjectT lymphocytes
dc.titleIFN-gamma production by CD8+ T cells depends on NFAT1 transcription factor and regulates Th differentiation
dc.typeArticle


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