| dc.creator | Bomfim, Barbara Cristina Marcollino | |
| dc.creator | Silva, Jessyca Azevedo | |
| dc.creator | Caminha, Giulia | |
| dc.creator | Santos, João Paulo Rodrigues | |
| dc.creator | Pelajo‑Machado, Marcelo | |
| dc.creator | Silva, Jackline de Paula Ayres | |
| dc.date | 2023-05-27T14:24:50Z | |
| dc.date | 2023-05-27T14:24:50Z | |
| dc.date | 2023 | |
| dc.date.accessioned | 2023-09-26T20:07:49Z | |
| dc.date.available | 2023-09-26T20:07:49Z | |
| dc.identifier | BOMFIM, Barbara Cristina Marcolino et al. Lectin‑based carbohydrate profile of megakaryocytes in murine fetal liver during development. Scientific Reports, v. 13-6729, p. 1 - 13, Apr. 2023. | |
| dc.identifier | 2045-2322 | |
| dc.identifier | https://www.arca.fiocruz.br/handle/icict/58771 | |
| dc.identifier | 10.1038/s41598-023-32863-3 | |
| dc.identifier.uri | https://repositorioslatinoamericanos.uchile.cl/handle/2250/8849228 | |
| dc.description | Hematopoiesis is the process by which blood cells are generated. During embryonic development,
these cells migrate through different organs until they reach the bone marrow, their definitive place
in adulthood. Around E10.5, the fetal liver starts budding from the gut, where first hematopoietic
cells arrive and expand. Hematopoietic cell migration occurs through cytokine stimulation,
receptor expression, and glycosylation patterns on the cell surface. In addition, carbohydrates can
modulate different cell activation states. For this reason, we aimed to characterize and quantify fetal
megakaryocytic cells in mouse fetal liver according to their glycan residues at different gestational
ages through lectins. Mouse fetuses between E11.5 and E18.5 were formalin-fixed and, paraffinembedded,
for immunofluorescence analysis using confocal microscopy. The results showed that
the following sugar residues were expressed in proliferating and differentiating megakaryocytes in
the fetal liver at different gestational ages: α-mannose, α-glucose, galactose, GlcNAc, and two types
of complex oligosaccharides. Megakaryocytes also showed three proliferation waves during liver
development at E12.5, E14.5, and E18.5. Additionally, the lectins that exhibited high and specific
pattern intensities at liver capsules and vessels were shown to be a less time-consuming and robust
alternative alternative to conventional antibodies for displaying liver structures such as capsules and
vessels, as well as for megakaryocyte differentiation in the fetal liver. | |
| dc.format | application/pdf | |
| dc.language | eng | |
| dc.publisher | Nature Portfolio | |
| dc.rights | open access | |
| dc.subject | Perfil de carboidratos à base de lectina | |
| dc.subject | Megacariócitos | |
| dc.subject | Durante o desenvolvimento | |
| dc.subject | Fígado de fetos murinos | |
| dc.subject | Lectin‑based carbohydrate profile | |
| dc.subject | Megakaryocytes | |
| dc.subject | Murine fetal liver | |
| dc.subject | During development | |
| dc.title | Lectin‑based carbohydrate profile of megakaryocytes in murine fetal liver during development | |
| dc.type | Article | |
