Detection of Trypanosoma cruzi in Blood Specimens of Chronic Chagasic Patients by Polymerase Chain Reaction Amplification of Kinetoplast Minicircle DNA: Comparison with Serology and Xenodiagnosis

dc.creatorAvila, Herbert A.
dc.creatorPereira, Jose Borges
dc.creatorThiemann, Otavio
dc.creatorPaiva, Eugenia de
dc.creatorDegrave, Wim
dc.creatorMorel, Carlos Medicis
dc.creatorSimpson, Larry
dc.date2016-06-29T14:58:50Z
dc.date2016-07-07T12:05:14Z
dc.date2016-06-29T14:58:50Z
dc.date2016-07-07T12:05:14Z
dc.date1993
dc.date.accessioned2023-09-26T20:07:47Z
dc.date.available2023-09-26T20:07:47Z
dc.identifierAVILA, Herbert A. et al. Detection of Trypanosoma cruzi in blood specimens of chronic chagasic patients by polymerase chain reaction amplification of kinetoplast minicircle DNA: comparison with serology and xenodiagnosis. Journal of Clinical Microbiology, v. 31, n. 9, p. 2421-2426, 1993.
dc.identifier0095-1137
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/14660
dc.identifier1098-660X
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8849212
dc.descriptionA panel of 114 blood samples from chronic chagasic patients and nonchagasic patients was screened for Trypanosoma cruzi by xenodiagnostic, serologic, and polymerase chain reaction (PCR) amplification tests. Blood samples were preserved in a guanidine-EDTA buffer, and total blood DNA was isolated after chemical nuclease cleavage with 1,10-phenanthroline-copper ion and used as a template for PCR amplification of the conserved and variable regions of T. cruzi minicircle molecules. The PCR products were screened by Southern blot hybridization with a digoxigenin-labeled oligonucleotide probe specific for the conserved region of the minicircle. The method showed a sensitivity of 100%o compared with the serologic test. In addition, all of the serology-positive, xenodiagnosis-negative samples were positive by PCR. This demonstrates that PCR amplification of T. cruzi kinetoplast minicircle DNA could replace xenodiagnosis for evaluation of parasitemia in chronic chagasic patients and could serve as a complement for serologic testing in the screening of blood bank donors.
dc.formatapplication/pdf
dc.languageeng
dc.publisherAmerican Society for Microbiology
dc.rightsopen access
dc.subjectTrypanosoma cruzi
dc.subjectChronic Chagasic Patients
dc.subjectPolymerase Chain Reaction
dc.subjectKinetoplast Minicircle DNA
dc.subjectSerology
dc.subjectXenodiagnosis
dc.subjectTrypanosoma cruzi
dc.subjectXenodiagnóstico
dc.subjectDoença de Chagas
dc.subjectReação em Cadeia da Polimerase
dc.subjectSorologia
dc.subjectDNA de Cinetoplasto
dc.titleDetection of Trypanosoma cruzi in Blood Specimens of Chronic Chagasic Patients by Polymerase Chain Reaction Amplification of Kinetoplast Minicircle DNA: Comparison with Serology and Xenodiagnosis
dc.typeArticle


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