The in vitro biological activity of the Brazilian brown seaweed Dictyota mertensii against Leishmania amazonensis

dc.creatorAliança, Amanda Silva dos Santos
dc.creatorAnjos, Keicyanne Fernanda Lessa dos
dc.creatorReis, Thiago Nogueira de Vasconcelos
dc.creatorHigino, Taciana Mirely Maciel
dc.creatorBrelaz-de-Castro, Maria Carolina Accioly
dc.creatorBianco, Éverson Miguel
dc.creatorFigueiredo, Regina Celia Bressan Queiroz de
dc.date2017-11-20T14:30:32Z
dc.date2017-11-20T14:30:32Z
dc.date2014
dc.date.accessioned2023-09-26T20:07:38Z
dc.date.available2023-09-26T20:07:38Z
dc.identifierALIANÇA, Amanda Silva dos S. et al. The in Vitro Biological Activity of the Brazilian Brown Seaweed Dictyota mertensii against Leishmania amazonensis. Molecules, v. 19, n. 9, p. 14052–14065, 9 set. 2014.
dc.identifier1420-3049
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/23272
dc.identifier10.3390/molecules190914052
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8849140
dc.descriptionSeaweeds present a wide variety of interesting bioactive molecules. In the present work we evaluated the biological activity of the dichloromethane/methanol (2:1) extract (DME) from the brown seaweed Dictyota mertensii against Leishmania amazonensis and its cytotoxic potential on mammalian cells. The extract showed significant inhibitory effect on the growth of promastigote forms (IC50=71.60 μg/mL) and low toxicity against mammalian cells (CC50=233.10 μg/mL). The DME was also efficient in inhibiting the infection in macrophages, with CC50 of 81.4 μg/mL and significantly decreased the survival of amastigote forms within these cells. The selectivity index showed that DME was more toxic to both promastigote (SI=3.25) and amastigote (SI=2.86) forms than to macrophages. Increased NO production was observed in treated macrophages suggesting that besides acting directly on the parasites, the DME also shows an immunomodulatory effect on macrophages. Drastic ultrastructural alterations consistent with loss of viability and cell death were observed in treated parasites. Confocal microscopy and cytometry analyzes showed no significant impairment of plasma membrane integrity, whereas an intense depolarization of mitochondrial membrane could be observed by using propidium iodide and rhodamine 123 staining, respectively. The low toxicity to mammalian cells and the effective activity against promastigotes and amastigotes, point to the use of DME as a promising agent for the treatment of cutaneous leishmaniasis.
dc.formatapplication/pdf
dc.languageeng
dc.rightsopen access
dc.subjectDictyota mertensii
dc.subjectquimioterapia
dc.subjectLeishmania amazonensi
dc.subjectultraestrutura
dc.subjectDictyota mertensii
dc.subjectchemotherapy
dc.subjectLeishmania amazonensi
dc.subjectultrastructure
dc.subjectAntiprotozoários
dc.subjectfarmacologia
dc.subjectLeishmania
dc.subjectefeitos de drogas
dc.subjectLeishmaniose
dc.subjecttratamento farmacológico
dc.subjectFeófitas
dc.subjectquímica
dc.subjectCélulas Cultivadas
dc.subjectanimais
dc.subjectAntiprotozoários
dc.subjectisolamento & purificação
dc.subjectAvaliação Pré-Clínica de Medicamentos
dc.subjectConcentração Inibidora 50
dc.subjectMacrófagos Peritoneais
dc.subjectmetabolismo
dc.titleThe in vitro biological activity of the Brazilian brown seaweed Dictyota mertensii against Leishmania amazonensis
dc.typeArticle


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