Biochemical characterization, action on macrophages, and superoxide anion production of four basic Phospholipases A2 from Panamanian Bothrops asper Snake Venom

dc.creatorRueda, Aristides Quintero
dc.creatorRodríguez, Isela González
dc.creatorArantes, Eliane C.
dc.creatorSetúbal, Sulamita S.
dc.creatorCalderon, Leonardo de A.
dc.creatorZuliani, Juliana P.
dc.creatorStábeli, Rodrigo G.
dc.creatorSoares, Andreimar M.
dc.date2016-07-14T15:25:25Z
dc.date2016-07-15T18:40:37Z
dc.date2016-07-14T15:25:25Z
dc.date2016-07-15T18:40:37Z
dc.date2013
dc.date.accessioned2023-09-26T20:07:32Z
dc.date.available2023-09-26T20:07:32Z
dc.identifierRUEDA, Aristides Quintero et al. Biochemical Characterization, Action on Macrophages, and Superoxide Anion Production of Four Basic Phospholipases A2 from Panamanian Bothrops asper Snake Venom. BioMed Research International, v. 2013, p. 1-9, 2013.
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/14946
dc.identifier10.1155/2013/789689
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8849101
dc.descriptionBothrops asper (Squamata: Viperidae) is the most important venomous snake in Central America, being responsible for the majority of snakebite accidents. Four basic PLA2s (pMTX-I to -IV) were purified from crude venom by a single-step chromatography using a CM-Sepharose ion-exchange column (1.5 × 15 cm). Analysis of the N-terminal sequence demonstrated that pMTX-I and III belong to the catalytically active Asp49 phospholipase A2 subclass, whereas pMTX-II and IV belong to the enzymatically inactive Lys49 PLA2s-like subclass. The PLA2s isolated from Panama Bothrops asper venom (pMTX-I, II, III, and IV) are able to induce myotoxic activity, inflammatory reaction mainly leukocyte migration to the muscle, and induce J774A.1 macrophages activation to start phagocytic activity and superoxide production.
dc.formatapplication/pdf
dc.languageeng
dc.publisherHindawi
dc.rightsopen access
dc.subjectBiochemical Characterization
dc.subjectMacrophages
dc.subjectSuperoxide Anion
dc.subjectPhospholipases A2
dc.subjectBothrops asper
dc.subjectSnake Venom
dc.titleBiochemical characterization, action on macrophages, and superoxide anion production of four basic Phospholipases A2 from Panamanian Bothrops asper Snake Venom
dc.typeArticle


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