Identification of Leishmania (Viannia) species and clinical isolates of Leishmania (Leishmania) amazonensis from Brazil using PCR-RFLP of the heat-shock protein 70 gene reveals some unexpected observations

dc.creatorEspada, Caroline Ricce
dc.creatorOrtiz, Paola Andrea
dc.creatorShaw, Jeffrey Jon
dc.creatorBarral, Aldina Maria Prado
dc.creatorCosta, Jackson Mauricio Lopes
dc.creatorUliana, Silvia Reni Bortolin
dc.creatorCoelho, Adriano Cappellazzo
dc.date2018-09-19T16:47:03Z
dc.date2018-09-19T16:47:03Z
dc.date2018
dc.date.accessioned2023-09-26T20:07:11Z
dc.date.available2023-09-26T20:07:11Z
dc.identifierESPADA, C. R. et al. Identification of Leishmania (Viannia) species and clinical isolates of Leishmania (Leishmania) amazonensis from Brazil using PCR-RFLP of the heat-shock protein 70 gene reveals some unexpected observations. Diagnostic Microbiology and Infectious Disease, v. 91, p. 312–318, 2018.
dc.identifier0732-8893
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/28899
dc.identifier10.1016/j.diagmicrobio.2018.03.004
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8848922
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP, 2011/20484-7 and 2015/09080-2) and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq, 473343/2012-6), Brazil. SRBU is the recipient of a senior researcher scholarship from CNPq. ACC and CRE were fellows supported by FAPESP (2012/14629-5 and 2016/23405-4).
dc.descriptionHsp70 is a cytoplasmic heat-shock protein, encoded by a multicopy tandemly repeated gene that has recently been gaining popularity as a valuable marker for typing Leishmania species. In this study, we used a previously described hsp70 PCR-RFLP method for identifying Brazilian Leishmania isolates. We identified two distinct L. (L.) amazonensis hsp70 alleles that resulted in two different RFLP patterns. Also, we found RFLP polymorphisms amongst L. (Viannia) naiffi strains. The profiles of both L. (V.) shawi and L. (V.) lindenbergi were very similar to those of other L. (Viannia) species. The observations described herein reflect the polymorphism found within species of Leishmania and indicate that results from this hsp70 PCR-RFLP method should be used with caution when typing isolates from clinical cases of leishmaniasis and Leishmania species from Brazil.
dc.formatapplication/pdf
dc.languageeng
dc.publisherElsevier
dc.rightsopen access
dc.subjectLeishmania (Leishmania) amazonensis
dc.subjectEspécie Leishmania (Viannia)
dc.subjecthsp70
dc.subjectPCR-RFLP
dc.subjectDiagnóstico molecular
dc.subjectLeishmania (Leishmania) amazonensis
dc.subjectLeishmania (Viannia) species
dc.subjecthsp70
dc.subjectPCR-RFLP
dc.subjectMolecular diagnosis
dc.titleIdentification of Leishmania (Viannia) species and clinical isolates of Leishmania (Leishmania) amazonensis from Brazil using PCR-RFLP of the heat-shock protein 70 gene reveals some unexpected observations
dc.typeArticle


Este ítem pertenece a la siguiente institución