Dual RNA-Seq of Human Leprosy Lesions Identifies Bacterial Determinants Linked to Host Immune Response

dc.creatorMontoya, Dennis J.
dc.creatorAndrade, Priscila
dc.creatorSilva, Bruno J. A.
dc.creatorTeles, Rosane M. B.
dc.creatorMa, Feiyang
dc.creatorBryson, Bryan
dc.creatorSadanand, Saheli
dc.creatorNoel, Teia
dc.creatorLu, Jing
dc.creatorSarno, Euzenir N.
dc.creatorArnvig, Kristine B.
dc.creatorYoung, Douglas
dc.creatorLahiri, Ramanuj
dc.creatorWilliams, Diana L.
dc.creatorFortune, Sarah
dc.creatorBloom, Barry R.
dc.creatorPellegrini, Matteo
dc.creatorModlin, Robert L.
dc.date2019-09-02T16:17:33Z
dc.date2019-09-02T16:17:33Z
dc.date2019
dc.date.accessioned2023-09-26T20:07:03Z
dc.date.available2023-09-26T20:07:03Z
dc.identifierMONTOYA, Dennis J. et al. Dual RNA-Seq of Human Leprosy Lesions Identifies Bacterial Determinants Linked to Host Immune Response. Cell Reports, v. 26, n. 13, p. 3574-3585, Mar. 2019.
dc.identifier2211-1247
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/35255
dc.identifier10.1016/j.celrep.2019.02.109
dc.identifier2211-1247
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8848871
dc.descriptionTo understand how the interaction between an intracellular bacterium and the host immune system contributes to outcome at the site of infection, we studied leprosy, a disease that forms a clinical spectrum, in which progressive infection by the intracellular bacterium Mycobacterium leprae is characterized by the production of type I IFNs and antibody production. Dual RNA-seq on patient lesions identifies two independent molecular measures of M. leprae, each of which correlates with distinct aspects of the host immune response. The fraction of bacterial transcripts, reflecting bacterial burden, correlates with a host type I IFN gene signature, known to inhibit antimicrobial responses. Second, the bacterial mRNA:rRNA ratio, reflecting bacterial viability, links bacterial heat shock proteins with the BAFF-BCMA host antibody response pathway. Our findings provide a platform for the interrogation of host and pathogen transcriptomes at the site of infection, allowing insight into mechanisms of inflammation in human disease.
dc.formatapplication/pdf
dc.languageeng
dc.publisherElsevier
dc.rightsopen access
dc.subjectHanseníase humana
dc.subjectRNA-Seq duplo
dc.subjectDeterminantes bacterianos
dc.subjectResposta imune do hospedeiro
dc.subjectMycobacterium leprae
dc.subjectHuman Leprosy
dc.subjectBacterial Determinants
dc.subjectHost Immune Response
dc.subjectDual RNA-Seq
dc.subjectMycobacterium leprae
dc.titleDual RNA-Seq of Human Leprosy Lesions Identifies Bacterial Determinants Linked to Host Immune Response
dc.typeArticle


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