Golgi Reassembly and Stacking Protein (GRASP) participates in vesicle-mediated RNA export in cryptococcus neoformans

dc.creatorSilva, Roberta Peres da
dc.creatorMartins, Sharon de Toledo
dc.creatorRizzo, Juliana
dc.creatorReis, Flavia Coelho Garcia dos
dc.creatorJoffe, Luna S.
dc.creatorVainstein, Marilene Henning
dc.creatorKmetzsch, Lívia
dc.creatorOliveira, Débora L.
dc.creatorPuccia, Rosana
dc.creatorGoldenberg, Samuel
dc.creatorRodrigues, Marcio Lourenço
dc.creatorAlves, Lysangela Ronalte
dc.date2018-11-13T16:37:36Z
dc.date2018-11-13T16:37:36Z
dc.date2018
dc.date.accessioned2023-09-26T20:07:02Z
dc.date.available2023-09-26T20:07:02Z
dc.identifierSILVA, Roberta Peres da et al. Golgi Reassembly and Stacking Protein (GRASP) participates in vesicle-mediated RNA export in cryptococcus neoformans. Genes, v. 9, n. 400, p. 1-17, 2018.
dc.identifier2073-4425
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/30015
dc.identifier10.3390/genes9080400
dc.identifier2073-4425
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8848861
dc.descriptionGolgi reassembly and stacking protein (GRASP) is required for polysaccharide secretion and virulence in Cryptococcus neoformans. In fungal species, extracellular vesicles (EVs) participate in the export of polysaccharides, proteins and RNA. In the present work, we investigated if EV-mediated RNA export is functionally connected with GRASP in C. neoformans using a graspΔ mutant. Since GRASP-mediated unconventional secretion involves autophagosome formation in yeast, we included the atg7Δ mutant with defective autophagic mechanisms in our analysis. All fungal strains exported EVs but deletion of GRASP or ATG7 profoundly affected vesicular dimensions. The mRNA content of the graspΔ EVs differed substantially from that of the other two strains. The transcripts associated to the endoplasmic reticulum were highly abundant transcripts in graspΔ EVs. Among non-coding RNAs (ncRNAs), tRNA fragments were the most abundant in both mutant EVs but graspΔ EVs alone concentrated 22 exclusive sequences. In general, our results showed that the EV RNA content from atg7Δ and WT were more related than the RNA content of graspΔ, suggesting that GRASP, but not the autophagy regulator Atg7, is involved in the EV export of RNA. This is a previously unknown function for a key regulator of unconventional secretion in eukaryotic cells.
dc.formatapplication/pdf
dc.languagepor
dc.publisherMDPI
dc.rightsopen access
dc.subjectExtracellular Vesicles
dc.subjectGolgi Apparatus
dc.subjectAutophagy-Related Protein 7
dc.subjectSecretory Pathway
dc.subjectARN
dc.subjectAparato de Golgi
dc.subjectProteína 7 Relacionada con la Autofagia
dc.subjectVías Secretoras
dc.subjectCryptococcus neoformans
dc.subjectRNA
dc.subjectVesículas Extracelulares
dc.subjectComplexo de Golgi
dc.subjectProteína 7 Relacionada à Autofagia
dc.subjectVia Secretória
dc.titleGolgi Reassembly and Stacking Protein (GRASP) participates in vesicle-mediated RNA export in cryptococcus neoformans
dc.typeArticle


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