Sulfonation and anticoagulant activity of botryosphaeran from Botryosphaeria rhodina MAMB-05 grown on fructose

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorMendes, Simone Ferreira
dc.creatordos Santos, Osvaldo
dc.creatorBarbosa, Aneli M.
dc.creatorVasconcelos, Ana Flora D.
dc.creatorAranda-Selverio, Gabriel
dc.creatorMonteiro, Nilson K.
dc.creatorDekker, Robert F. H.
dc.creatorPereira, Mariana Sa
dc.creatorTovar, Ana Maria F.
dc.creatorde Souza Mourao, Paulo A.
dc.creatorCorradi da Silva, Maria de Lourdes
dc.date2014-05-20T15:33:01Z
dc.date2016-10-25T18:09:26Z
dc.date2014-05-20T15:33:01Z
dc.date2016-10-25T18:09:26Z
dc.date2009-10-01
dc.date.accessioned2017-04-06T00:30:45Z
dc.date.available2017-04-06T00:30:45Z
dc.identifierInternational Journal of Biological Macromolecules. Amsterdam: Elsevier B.V., v. 45, n. 3, p. 305-309, 2009.
dc.identifier0141-8130
dc.identifierhttp://hdl.handle.net/11449/41760
dc.identifierhttp://acervodigital.unesp.br/handle/11449/41760
dc.identifier10.1016/j.ijbiomac.2009.06.004
dc.identifierWOS:000269393500017
dc.identifierhttp://dx.doi.org/10.1016/j.ijbiomac.2009.06.004
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/884440
dc.descriptionBotryosphaeran (EPS(FRU)), an exopolysaccharide of the beta-(1 -> 3,1 -> 6)-D-glucan type with 31% branching at C-6, is produced by the fungus Botryosphaeria rhodina MAMB-05 when grown on fructose as carbon source. Botryosphaeran was derivatized by sulfonation to induce anticoagulant activity. The effectiveness of the sulfonation reaction by chlorosulfonic acid in pyridine was monitored by the degree of substitution and FT-IR analysis of the sulfonated EPS(FRU) (once sulfonated, EPS(FRU SULF); and re-sulfonated, EPS(FRU RESULF)). Activated partial thromboplastin time (APTT) and thrombin time (TT) tests of EPS(FRU RESULF) indicated significant in vitro anticoagulant activity that was dose-dependent. EPS(FRU) did not inhibit any of the coagulation tests. (C) 2009 Elsevier B.V. All rights reserved.
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.languageeng
dc.publisherElsevier B.V.
dc.relationInternational Journal of Biological Macromolecules
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectBotryosphaeria rhodina MAMB-05
dc.subjectExopolysaccharide
dc.subjectbeta-(1 -> 3,1 -> 6)-D-Glucan
dc.subjectSulfonation
dc.subjectAnticoagulant activity
dc.titleSulfonation and anticoagulant activity of botryosphaeran from Botryosphaeria rhodina MAMB-05 grown on fructose
dc.typeOtro


Este ítem pertenece a la siguiente institución