dc.contributor | Universidade Estadual Paulista (UNESP) | |
dc.creator | de Souza, Renato Aparecido | |
dc.creator | Xavier, Murilo | |
dc.creator | da Silva, Fabiano Fernandes | |
dc.creator | de Souza, Marco Tulio | |
dc.creator | Tosato, Maira Gaspar | |
dc.creator | Martin, Airton Abrahao | |
dc.creator | de Melo Castilho, Julio Cezar | |
dc.creator | Ribeiro, Wellington | |
dc.creator | Silveira, Landulfo | |
dc.date | 2014-05-20T15:31:36Z | |
dc.date | 2016-10-25T18:07:29Z | |
dc.date | 2014-05-20T15:31:36Z | |
dc.date | 2016-10-25T18:07:29Z | |
dc.date | 2012-03-01 | |
dc.date.accessioned | 2017-04-06T00:23:02Z | |
dc.date.available | 2017-04-06T00:23:02Z | |
dc.identifier | Lasers In Medical Science. London: Springer London Ltd, v. 27, n. 2, p. 487-495, 2012. | |
dc.identifier | 0268-8921 | |
dc.identifier | http://hdl.handle.net/11449/40690 | |
dc.identifier | http://acervodigital.unesp.br/handle/11449/40690 | |
dc.identifier | 10.1007/s10103-011-0976-0 | |
dc.identifier | WOS:000300588500031 | |
dc.identifier | http://dx.doi.org/10.1007/s10103-011-0976-0 | |
dc.identifier.uri | http://repositorioslatinoamericanos.uchile.cl/handle/2250/883440 | |
dc.description | The influence of creatine (Cr) supplementation on cortical and trabecular bone from ovariectomized rats was studied using FT-Raman spectroscopy. The intensity of organic-phase Raman bands was compared to mineral phase ones. Twenty-one female Wistar rats aged 3 months were divided into three groups (n = 7 per group): ovariectomized (OVX), ovariectomized treated with creatine (CRE) and sham-operated (SHAM) groups. Creatine supplementation (300 mg kg(-1) day(-1)) was provided for 8 weeks, starting 12 weeks after ovariectomy. FT-Raman spectroscopy was performed on the right medial femoral mid-shaft (cortical bone) and third lumbar vertebral body (trabecular bone). The integrated intensities of mineral phase (phosphate and carbonate bands at 959 and 1,071 cm(-1), respectively) and organic phase (amide I band at 1,665 cm(-1)) Raman bands were analyzed. The mineral-to-matrix (phosphate/amide I), carbonate-to-phosphate, and carbonate-to-amide I ratios were analyzed to assess bone quality. The phosphate content on trabecular bone was higher in the CRE group than the OVX group (p < 0.05). No significant changes in mineral or organic phases on cortical bone were observed. A radiographic assessment of bone density was encouraging as the same findings were showed by Raman intensity of phosphate from cortical (r (2) = 0.8037) and trabecular bones (r (2) = 0.915). Severe ovariectomy-induced bone loss was confirmed by FT-Raman spectroscopy. The results suggest that the chemical composition of trabecular bone tissue may be positively influenced by Cr supplementation after ovariectomy. | |
dc.description | Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) | |
dc.language | eng | |
dc.publisher | Springer London Ltd | |
dc.relation | Lasers in Medical Science | |
dc.rights | info:eu-repo/semantics/closedAccess | |
dc.subject | Cortical bone | |
dc.subject | Nutritional supplement | |
dc.subject | Optical spectroscopy | |
dc.subject | Osteoporosis | |
dc.subject | Trabecular bone | |
dc.title | Influence of creatine supplementation on bone quality in the ovariectomized rat model: an FT-Raman spectroscopy study | |
dc.type | Otro | |