Influence of creatine supplementation on bone quality in the ovariectomized rat model: an FT-Raman spectroscopy study

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorde Souza, Renato Aparecido
dc.creatorXavier, Murilo
dc.creatorda Silva, Fabiano Fernandes
dc.creatorde Souza, Marco Tulio
dc.creatorTosato, Maira Gaspar
dc.creatorMartin, Airton Abrahao
dc.creatorde Melo Castilho, Julio Cezar
dc.creatorRibeiro, Wellington
dc.creatorSilveira, Landulfo
dc.date2014-05-20T15:31:36Z
dc.date2016-10-25T18:07:29Z
dc.date2014-05-20T15:31:36Z
dc.date2016-10-25T18:07:29Z
dc.date2012-03-01
dc.date.accessioned2017-04-06T00:23:02Z
dc.date.available2017-04-06T00:23:02Z
dc.identifierLasers In Medical Science. London: Springer London Ltd, v. 27, n. 2, p. 487-495, 2012.
dc.identifier0268-8921
dc.identifierhttp://hdl.handle.net/11449/40690
dc.identifierhttp://acervodigital.unesp.br/handle/11449/40690
dc.identifier10.1007/s10103-011-0976-0
dc.identifierWOS:000300588500031
dc.identifierhttp://dx.doi.org/10.1007/s10103-011-0976-0
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/883440
dc.descriptionThe influence of creatine (Cr) supplementation on cortical and trabecular bone from ovariectomized rats was studied using FT-Raman spectroscopy. The intensity of organic-phase Raman bands was compared to mineral phase ones. Twenty-one female Wistar rats aged 3 months were divided into three groups (n = 7 per group): ovariectomized (OVX), ovariectomized treated with creatine (CRE) and sham-operated (SHAM) groups. Creatine supplementation (300 mg kg(-1) day(-1)) was provided for 8 weeks, starting 12 weeks after ovariectomy. FT-Raman spectroscopy was performed on the right medial femoral mid-shaft (cortical bone) and third lumbar vertebral body (trabecular bone). The integrated intensities of mineral phase (phosphate and carbonate bands at 959 and 1,071 cm(-1), respectively) and organic phase (amide I band at 1,665 cm(-1)) Raman bands were analyzed. The mineral-to-matrix (phosphate/amide I), carbonate-to-phosphate, and carbonate-to-amide I ratios were analyzed to assess bone quality. The phosphate content on trabecular bone was higher in the CRE group than the OVX group (p < 0.05). No significant changes in mineral or organic phases on cortical bone were observed. A radiographic assessment of bone density was encouraging as the same findings were showed by Raman intensity of phosphate from cortical (r (2) = 0.8037) and trabecular bones (r (2) = 0.915). Severe ovariectomy-induced bone loss was confirmed by FT-Raman spectroscopy. The results suggest that the chemical composition of trabecular bone tissue may be positively influenced by Cr supplementation after ovariectomy.
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.languageeng
dc.publisherSpringer London Ltd
dc.relationLasers in Medical Science
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectCortical bone
dc.subjectNutritional supplement
dc.subjectOptical spectroscopy
dc.subjectOsteoporosis
dc.subjectTrabecular bone
dc.titleInfluence of creatine supplementation on bone quality in the ovariectomized rat model: an FT-Raman spectroscopy study
dc.typeOtro


Este ítem pertenece a la siguiente institución