Parentage testing in alpacas (Vicugna pacos) using semi-automated fluorescent multiplex PCRs with 10 microsatellite markers

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorAgapito, J.
dc.creatorRodriguez, J.
dc.creatorHerrera-Velit, P.
dc.creatorTimoteo, O.
dc.creatorRojas, P.
dc.creatorBoettcher, P. J.
dc.creatorGarcia, F.
dc.creatorEspinoza, J. R.
dc.date2014-05-20T15:31:14Z
dc.date2016-10-25T18:07:00Z
dc.date2014-05-20T15:31:14Z
dc.date2016-10-25T18:07:00Z
dc.date2008-04-01
dc.date.accessioned2017-04-06T00:21:11Z
dc.date.available2017-04-06T00:21:11Z
dc.identifierAnimal Genetics. Oxford: Blackwell Publishing, v. 39, n. 2, p. 201-203, 2008.
dc.identifier0268-9146
dc.identifierhttp://hdl.handle.net/11449/40429
dc.identifierhttp://acervodigital.unesp.br/handle/11449/40429
dc.identifier10.1111/j.1365-2052.2007.01697.x
dc.identifierWOS:000254381100015
dc.identifierhttp://dx.doi.org/10.1111/j.1365-2052.2007.01697.x
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/883204
dc.descriptionThe aim of this study was to assess and apply a microsatellite multiplex system for parentage determination in alpacas. An approach for parentage testing based on 10 microsatellites was evaluated in a population of 329 unrelated alpacas from different geographical zones in Peru. All microsatellite markers, which amplified in two multiplex reactions, were highly polymorphic with a mean of 14.5 alleles per locus (six to 28 alleles per locus) and an average expected heterozygosity (H-E) of 0.8185 (range of 0.698-0.946). The total parentage exclusion probability was 0.999456 for excluding a candidate parent from parentage of an arbitrary offspring, given only the genotype of the offspring, and 0.999991 for excluding a candidate parent from parentage of an arbitrary offspring, given the genotype of the offspring and the other parent. In a case test of parentage assignment, the microsatellite panel assigned 38 (from 45 cases) offspring parentage to 10 sires with LOD scores ranging from 2.19 x 10(+13) to 1.34 x 10(+15) and Delta values ranging from 2.80 x 10(+12) to 1.34 x 10(+15) with an estimated pedigree error rate of 15.5%. The performance of this multiplex panel of markers suggests that it will be useful in parentage testing of alpacas.
dc.languageeng
dc.publisherBlackwell Publishing
dc.relationAnimal Genetics
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectalpaca
dc.subjectgenotyping
dc.subjectmicrosatellites
dc.subjectmultiplex
dc.subjectparentage
dc.titleParentage testing in alpacas (Vicugna pacos) using semi-automated fluorescent multiplex PCRs with 10 microsatellite markers
dc.typeOtro


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