Analysis of the activity of oncocalyxone A (Auxemma oncocalyx) and doxorubicin on the in vitro development of porcine oocytes

dc.creatorRevilla,Johanna Leiva
dc.creatorMaside,Carolina
dc.creatorVieira,Luis
dc.creatorCadenas,Jesús
dc.creatorAcioly,Ana Clara Ferreira
dc.creatorPaes,Victor Macedo
dc.creatorAgiar,Francisco Leo
dc.creatorCelestino,Juliana Jales de Hollanda
dc.creatorAlves,Benner Geraldo
dc.creatorPessoa,Otilia Deusdenia Loiola
dc.creatorToniolli,Ricardo
dc.creatorRodrigues,Ana Paula
dc.creatorFigueiredo,José Ricardo de
dc.date2019-12-01
dc.date.accessioned2023-09-25T15:51:25Z
dc.date.available2023-09-25T15:51:25Z
dc.identifierhttp://scielo.iics.una.py/scielo.php?script=sci_arttext&pid=S2617-47312019000200274
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8831314
dc.descriptionABSTRACT Most anticancer drugs like doxorubicin (DXR) have low specificity that results in undesirable effects especially when it comes to collateral effects on reproduction. Plants are excellent sources when searching for new drugs. Auxemma oncocalyx (A. oncocalyx) and its main component Oncocalyxone A (onco A) have anti-tumoral activity and are less toxic than DXR in reproductive parameters. However, there are no studies on the action of these drugs regarding the porcine in vitro oocyte competence and embryo development. The aim of this study was to evaluate the effect of A. oncocalyx and onco A exposure during in vitro maturation (IVM) of oocytes (Experiment 1) or in vitro embryo culture (IVC) (Experiment 2) on the oocyte developmental competence. For experiment 1, COCs were distributed in IVM medium alone (control) or supplemented with DXR (0.3 (g/mL), A. oncocalyx (1.2 (g/mL) and onco A (1 (g/mL). Then, oocytes were submitted to in vitro fertilization (IVF) and in vitro embryo culture. For experiment 2, zygotes were cultured with DXR, A. oncocalyx and onco A for 7 days. Viability, maturation, fertilization and embryo developmental parameters were evaluated in both experiments. In experiment 1; DXR, A. oncocalyx and onco A reduced (P<0.05) oocyte viability and IVM efficiency. Onco A increased (P<0.05) the meiotic resumption. After IVF, all drugs reduced (P<0.05) viability, IVF efficiency and percentage of cleaved embryos, nevertheless, only DXR decreased the percentage of blastocyst. In experiment 2; all drugs reduced (P<0.05) the percentage of penetration, but only DXR and onco A decreased (P<0.05) IVF efficiency. DXR and A. oncocalyx decreased (P<0.05) the percentage of cleaved embryo, but had no effect on blastocyst formation. In conclusion, the addition of DXR during IVM or IVC negatively affected the IVF efficiency and cleavage rate. In addition, the exposure of COCs to DXR only during IVM was more detrimental to oocyte viability and blastocyst formation than A. oncocalyx and onco A.
dc.formattext/html
dc.languageen
dc.publisherSociedad Científica del Paraguay
dc.relation10.32480/rscp.2019-24-2.274-292
dc.rightsinfo:eu-repo/semantics/openAccess
dc.sourceRevista de la Sociedad Científica del Paraguay v.24 n.2 2019
dc.subjectAuxemma oncocalyx
dc.subjectOncocalyxone A
dc.subjectDoxorubicin
dc.subjectin vitro maturation
dc.subjectin vitro fertilization
dc.subjectCOCs
dc.subjectembryo development
dc.titleAnalysis of the activity of oncocalyxone A (Auxemma oncocalyx) and doxorubicin on the in vitro development of porcine oocytes
dc.typeinfo:eu-repo/semantics/article


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