Progesterone upregulates GATA-1 on erythroid progenitors cells in liquid culture

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorDuarte, ADS
dc.creatorSales, TSI
dc.creatorMengel, J. O.
dc.creatorCosta, F. F.
dc.creatorSaad, STO
dc.date2014-05-20T15:30:43Z
dc.date2016-10-25T18:06:19Z
dc.date2014-05-20T15:30:43Z
dc.date2016-10-25T18:06:19Z
dc.date2002-09-01
dc.date.accessioned2017-04-06T00:18:31Z
dc.date.available2017-04-06T00:18:31Z
dc.identifierBlood Cells Molecules and Diseases. San Diego: Academic Press Inc. Elsevier B.V., v. 29, n. 2, p. 213-224, 2002.
dc.identifier1079-9796
dc.identifierhttp://hdl.handle.net/11449/40040
dc.identifierhttp://acervodigital.unesp.br/handle/11449/40040
dc.identifier10.1006/bcmd.2002.0551
dc.identifierWOS:000179991100009
dc.identifierhttp://dx.doi.org/10.1006/bcmd.2002.0551
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/882854
dc.descriptionSteroids hormones modify the hematological features of homozygous sickle cell disease, including the levels of fetal hemoglobin. We used semi-quantitative RT-PCR analysis of GATA-1, GATA-2, NF-E2, and gamma-globin mRNA levels in a two-phase liquid culture system of human adult erythroid cells in order to assay the effect of progesterone upon gene expression. The levels of expression of GATA-1 and gamma-globin mRNA were significantly increased in cells treated with progesterone compared to untreated cells (1.7- to 2.0-fold). Progesterone treatment did not produce any stimulatory effect upon GATA-2 and NF-E2 mRNA expression. Differences in the synthesis of HbF protein could not be detected by flow cytometry, although we observed a small difference in mean intensity fluorescence between cells treated and cells untreated with progesterone on days 7 and 9. Using anti-transferrin receptor and anti-glycophorin A antibodies, we verified that addition of progesterone did not cause any change in erythroid proliferation and differentiation. In conclusion, it is possible that the increased expression of gamma-globin mRNA after progesterone treatment observed in this study may be related to the increased GATA-1 mRNA expression. Interactions of the steroid receptors with the basal transcriptional machinery and with transcription factors might mediate their transcriptional effects. (C) 2002 Elsevier B.V. (USA).
dc.languageeng
dc.publisherElsevier B.V.
dc.relationBlood Cells Molecules and Diseases
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectHbF
dc.subjectprogesterone
dc.subjecttranscription factors
dc.subjectRT-PCR
dc.subjectgene expression
dc.titleProgesterone upregulates GATA-1 on erythroid progenitors cells in liquid culture
dc.typeOtro


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