Fungicidal activity of human monocyte-derived multinucleated giant cells induced in vitro by Paracoccidioides brasiliensis antigen

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorPereira do Nascimento, Magda Paula
dc.creatorSoares, Ângela Maria Victoriano de Campos
dc.creatorDias-Melicio, Luciane Alarcao
dc.creatorParise-Fortes, Maria Rita
dc.creatorRodrigues Martins, Rosana Aparecida
dc.creatorNakaira, Erika Takahashi
dc.creatorSerrao Peracoil, Maria Terezinha
dc.date2014-05-20T15:30:01Z
dc.date2016-10-25T18:05:23Z
dc.date2014-05-20T15:30:01Z
dc.date2016-10-25T18:05:23Z
dc.date2008-07-01
dc.date.accessioned2017-04-06T00:14:42Z
dc.date.available2017-04-06T00:14:42Z
dc.identifierMycopathologia. Dordrecht: Springer, v. 166, n. 1, p. 25-33, 2008.
dc.identifier0301-486X
dc.identifierhttp://hdl.handle.net/11449/39466
dc.identifierhttp://acervodigital.unesp.br/handle/11449/39466
dc.identifier10.1007/s11046-007-9051-6
dc.identifierWOS:000257215400003
dc.identifierhttp://dx.doi.org/10.1007/s11046-007-9051-6
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/882364
dc.descriptionMultinucleated giant cells (MGC) are characteristic cells in granulomatous disorders such as paracoccidioidomycosis (PCM) and also are formed in vitro from peripheral blood mononuclear cells by several stimuli. In this study, the authors investigated in vitro formation of MGC derived from monocytes of healthy individuals, stimulated with Paracoccidioides brasiliensis antigen (PbAg), compared with other stimuli such as IFN-gamma and supernatant of Con-A-stimulated peripheral blood mononuclear cells (CM-ConA). Besides, the fungicidal activity of monocytes and monocyte-derived MGC challenged with P. brasiliensis were compared, at a ratio of one fungus per 50 monocytes. Results demonstrated that PbAg, IFN-gamma, and CM-ConA stimuli were able to induce MGC generation, with fusion indices significantly higher than control cultures. Striking results were observed when MGC induced by PbAg and IFN-gamma presented higher fungicidal activity than monocytes, submitted to the same stimuli, showing a better capacity of these cells to kill P. brasiliensis. In summary, the results suggest that PbAg is able to induce MGC generation, and these cells presented higher fungicidal activity against P. brasiliensis than monocytes.
dc.languageeng
dc.publisherSpringer
dc.relationMycopathologia
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectfungicidal activity
dc.subjectmultinucleated giant cells
dc.subjectparacoccidioides brasiliensis
dc.subjectinterferon-gamma
dc.titleFungicidal activity of human monocyte-derived multinucleated giant cells induced in vitro by Paracoccidioides brasiliensis antigen
dc.typeOtro


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