| dc.contributor | Universidade Estadual Paulista (UNESP) | |
| dc.creator | de Souza, C. C. | |
| dc.creator | Pellizzon, C. H. | |
| dc.creator | Hiraishi, M. | |
| dc.creator | Goldman, MHS | |
| dc.creator | Goldman, G. H. | |
| dc.date | 2014-05-20T15:29:59Z | |
| dc.date | 2016-10-25T18:05:20Z | |
| dc.date | 2014-05-20T15:29:59Z | |
| dc.date | 2016-10-25T18:05:20Z | |
| dc.date | 1998-01-01 | |
| dc.date.accessioned | 2017-04-06T00:14:30Z | |
| dc.date.available | 2017-04-06T00:14:30Z | |
| dc.identifier | Current Genetics. New York: Springer, v. 33, n. 1, p. 60-69, 1998. | |
| dc.identifier | 0172-8083 | |
| dc.identifier | http://hdl.handle.net/11449/39440 | |
| dc.identifier | http://acervodigital.unesp.br/handle/11449/39440 | |
| dc.identifier | 10.1007/s002940050309 | |
| dc.identifier | WOS:000072146000010 | |
| dc.identifier | http://dx.doi.org/10.1007/s002940050309 | |
| dc.identifier.uri | http://repositorioslatinoamericanos.uchile.cl/handle/2250/882340 | |
| dc.description | Aspergillus nidulans is a non-pathogenic fungus with well-developed genetics which provides an excellent model system for studying different aspects of drug resistance in filamentous fungi. As a preliminary step to characterizing genes that confer pleiotropic drug resistance in Aspergillus, we isolated cycloheximide-sensitive mutants of A. nidulans, which is normally resistant to this: drug. The rationale for this approach is to identify gents whose products are important for drug resistance by analysing mutations that alter the resistance/sensitivity status of the cell. Fifteen cycloheximide-sensitive (named scy for sensitive to cycloheximide) mutants of A, nidulans were isolated and genetically characterised. Each scy mutant was crossed with the wild-type strain and five of the crosses gave 50% cycloheximide-sensitive progeny suggesting that they carry a single mutation required for cycloheximide sensitivity. We examined ten sep mutants for resistance/sensitivity to other drugs or stress agents with different and/or the same mechanism of action, Sis of these mutants exhibited other altered resistance/sensitivity phenotypes which were linked to the cycloheximide sensitivity, These six mutants were analyzed by pairwise crosses and found to represent six linkage groups, named scyA-F. One of the mutants showed fragmentation of its vacuolar system and, in addition, its growth was osmotic, low-pi-II and oxidative-stress sensitive. | |
| dc.language | eng | |
| dc.publisher | Springer | |
| dc.relation | Current Genetics | |
| dc.rights | info:eu-repo/semantics/closedAccess | |
| dc.subject | cycloheximide-sensitive mutants | |
| dc.subject | Aspergillus nidulans | |
| dc.subject | pleitropic drug resistance | |
| dc.subject | vacuoles | |
| dc.title | Isolation and characterisation of cycloheximide sensitive mutants of Aspergillus nidulans | |
| dc.type | Otro | |
