| dc.creator | Segura-Ulate,Ismael | |
| dc.creator | Bolívar-González,Alejandro | |
| dc.creator | Madrigal-Redondo,Germán | |
| dc.creator | Nuñez-Corrales,Santiago | |
| dc.creator | Gatica-Arias,Andrés | |
| dc.date | 2022-12-01 | |
| dc.date.accessioned | 2023-09-25T14:33:48Z | |
| dc.date.available | 2023-09-25T14:33:48Z | |
| dc.identifier | http://www.scielo.sa.cr/scielo.php?script=sci_arttext&pid=S0034-77442022000100173 | |
| dc.identifier.uri | https://repositorioslatinoamericanos.uchile.cl/handle/2250/8822232 | |
| dc.description | Abstract Introduction: Most successful cases of COVID-19 pandemic mitigation and handling have relied on extensive reverse-transcription quantitative polymerase chain reaction (RT-qPCR). However, many emerging economies have struggled with current molecular testing demands due to economic, technical and technological constraints. Objective: To define a potential diagnostic protocol to increase testing capacity in current and post-pandemic conditions. Methods: We reviewed the literature, patents and commercial applications, for alternatives. Results: We found a good potential in saliva samples, viral inactivation and quick RNA extraction by heating; the use of an isothermal technology such as loop mediated isothermal amplification (LAMP) and naked eye test-result visualization by in-tube colorimetry or turbidity. Conclusions: Saliva samples with quick RNA extraction by heating and colorimetric LAMP are promising options for countries with economic and infrastructure limitations. | |
| dc.format | text/html | |
| dc.language | en | |
| dc.publisher | Universidad de Costa Rica | |
| dc.relation | 10.15517/rev.biol.trop..v70i1.47407 | |
| dc.rights | info:eu-repo/semantics/openAccess | |
| dc.source | Revista de Biología Tropical v.70 n.1 2022 | |
| dc.subject | emerging economies | |
| dc.subject | molecular testing | |
| dc.subject | SARS-CoV-2 | |
| dc.subject | saliva | |
| dc.subject | loop mediated isothermal amplification. | |
| dc.title | Reverse-Transcription Loop-Mediated Isothermal Amplification and alternative protocols for lower cost, large-scale COVID-19 testing: lessons from an emerging economy | |
| dc.type | info:eu-repo/semantics/article | |
