| dc.creator | Rostelato-Ferreira,Sandro | |
| dc.creator | Vettorazzo,Orlando-B. | |
| dc.creator | Tribuiani,Natália | |
| dc.creator | Leal,Allan-P. | |
| dc.creator | Dal-Belo,Cháriston-A. | |
| dc.creator | Rodrigues-Simioni,Léa | |
| dc.creator | Floriano,Rafael-S. | |
| dc.creator | Oshima-Franco,Yoko | |
| dc.date | 2021-06-01 | |
| dc.date.accessioned | 2023-09-25T14:23:58Z | |
| dc.date.available | 2023-09-25T14:23:58Z | |
| dc.identifier | http://www.scielo.sa.cr/scielo.php?script=sci_arttext&pid=S0034-77442021000200545 | |
| dc.identifier.uri | https://repositorioslatinoamericanos.uchile.cl/handle/2250/8819080 | |
| dc.description | Abstract Introduction: Rhinella schneideri is a toad widely distributed in South America and its poison is characterized by inducing cardiotoxicity and neurotoxicity. Objective: In this work, we investigated pharmacological strategies to attenuate the peripheral neurotoxicity induced by R. schneideri poison in avian neuromuscular preparation. Methods: The experiments were carried out using isolated chick biventer cervicis preparation subjected to field stimulation for muscle twitches recordings or exposed to acetylcholine and potassium chloride for contracture responses. Results: Poison (10 μg/ml) produced complete neuromuscular blockade in chick biventer cervicis preparation within approximately 70 min incubation (times for 50 and 90 % blockade: 15 ± 3 min and 40 ± 2 min, respectively; P < 0.05, N= 5); contracture responses to exogenous acetylcholine and KCl were unaffected by poison indicating no specificity with postsynaptic receptors or myotoxicity, respectively. Poison (10 μg/ml)-induced neuromuscular blockade was not prevented by heparin (5 and 150 IU/ml) under pre- or post-treatment conditions. Incubation at low temperature (23-25 °C) abolished the neuromuscular blockade; after raising the temperature to 37 °C, the complete neuromuscular blockade was slightly slower than that seen in preparations directly incubated at 37 °C (times for 50 and 90 % blockade: 23 ± 2 min and 60 ± 2.5 min, respectively; P < 0.05, N= 4). Neostigmine (3.3 μM) did not reverse the neuromuscular blockade in BC preparation whereas 3,4-diaminopyridine (91.6 μM) produced a partial and sustained reversal of the twitch responses (29 ± 7.8 % of maximal reversal reached in approximately 40 min incubation; P < 0.05, N= 4). Conclusions: R. schneideri poison induces potent peripheral neurotoxicity in vitro which can be partially reversible by 3,4-diaminopyridine. | |
| dc.format | text/html | |
| dc.language | en | |
| dc.publisher | Universidad de Costa Rica | |
| dc.relation | 10.15517/rbt.v69i2.44539 | |
| dc.rights | info:eu-repo/semantics/openAccess | |
| dc.source | Revista de Biología Tropical v.69 n.2 2021 | |
| dc.subject | amphibian poison | |
| dc.subject | paratoid gland secretion | |
| dc.subject | chick biventer cervicis preparation | |
| dc.subject | neuromuscular blockade | |
| dc.subject | reversal | |
| dc.title | Action of heparin and acetylcholine modulators on the neurotoxicity of the toad Rhinella schneideri (Anura: Bufonidae) in Brazil | |
| dc.type | info:eu-repo/semantics/article | |
