Genetic authentication of Eclipta prostrate (Asteraceae) from Penthorum chinense (Penthoraceae) by Sequence Characterized Amplified Region (SCAR) markers

dc.creatorMei,Zhiqiang
dc.creatorKhan,Md.-Asaduzzaman
dc.creatorFu,Junjiang
dc.date2020-03-01
dc.date.accessioned2023-09-25T14:21:44Z
dc.date.available2023-09-25T14:21:44Z
dc.identifierhttp://www.scielo.sa.cr/scielo.php?script=sci_arttext&pid=S0034-77442020000100180
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8818335
dc.descriptionAbstract Introduction: For the rapid and accurate genetic identification and authentication of living organisms, improved random amplified polymorphic DNA (RAPD) fragment based development of sequence-characterized amplified region (SCAR) markers is an important genetic technique. Objective: This study aimed to develop SCAR markers for perennial herb Eclipta prostrate (E. prostrate). Methods: Here the RAPD fragments by improved RAPD amplification with primers A11 and N-7 for E. prostrate were cloned into pGEX-T vector, and PCR amplification identified the positive clones. After the enzymatic digestion, they were sequenced with Sanger sequencing. Results: Two SCAR markers were developed, which were very specific to E. prostrate, not found in Penthorum chinense Pursh(P. chinense). The nucleotide sequence search by BLAST GenBank database showed that they are novel in E. prostrate, therefore they were deposited in Genbank with accession number KX671034, KX671035. The markers did not show any identity to other species. Conclusions: Thus, in this study two specific SCAR markers were developed for genetically distinguishing and identifying the plant species E. prostrate from herb P. chinense and others.
dc.formattext/html
dc.languageen
dc.publisherUniversidad de Costa Rica
dc.relation10.15517/rbt.v68i1.37046
dc.rightsinfo:eu-repo/semantics/openAccess
dc.sourceRevista de Biología Tropical v.68 n.1 2020
dc.subjectEclipta prostrate
dc.subjectgenetic authentication
dc.subjectPenthorum chinense Pursh
dc.subjectrandom amplified polymorphic DNA
dc.subjectsequence characterized amplified region
dc.titleGenetic authentication of Eclipta prostrate (Asteraceae) from Penthorum chinense (Penthoraceae) by Sequence Characterized Amplified Region (SCAR) markers
dc.typeinfo:eu-repo/semantics/article


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