| dc.creator | Vega,Rafael | |
| dc.creator | Vásquez,Nelly | |
| dc.creator | Espinoza,Ana M | |
| dc.creator | Gatica,Andrés M | |
| dc.creator | Valdez-Melara,Marta | |
| dc.date | 2009-11-01 | |
| dc.date.accessioned | 2023-09-25T14:01:58Z | |
| dc.date.available | 2023-09-25T14:01:58Z | |
| dc.identifier | http://www.scielo.sa.cr/scielo.php?script=sci_arttext&pid=S0034-77442009000500013 | |
| dc.identifier.uri | https://repositorioslatinoamericanos.uchile.cl/handle/2250/8810947 | |
| dc.description | Rice (Oryza sativa cv. 5272) embryogenic calli were obtained from mature zygotic embryos culture on Murashige & Skoog (1962) medium supplemented with 2.5 mg/l 2,4- dichlorophenoxyacetic acid. Histological analysis of somatic embryogenesis revealed that after two weeks of culture of explants on the callus induction medium, somatic embryo development began with a cluster of proembryogenic cells in the peripheral region of the calli. The outer cell layer of embryogenic calli consisted of small and isodiametric cells with a dense cytoplasm and a prominent nucleus and nucleolus; whereas the inner cell layer is composed of large cells with small nucleus and large vacuole. These embryogenic cells underwent a series of organized divisions and formed the proembryo with a well-defined protodermis. Rev. Biol. Trop. 57 (Suppl. 1): 141-150. Epub 2009 November 30. | |
| dc.format | text/html | |
| dc.language | en | |
| dc.publisher | Universidad de Costa Rica | |
| dc.rights | info:eu-repo/semantics/openAccess | |
| dc.source | Revista de Biología Tropical v.57 suppl.1 2009 | |
| dc.subject | Oryza sativa | |
| dc.subject | rice | |
| dc.subject | in vitro culture | |
| dc.subject | histology | |
| dc.subject | somatic embryogenesis | |
| dc.subject | morphogenesis | |
| dc.subject | Costa Rica | |
| dc.title | Histology of somatic embryogenesis in rice (Oryza sativa cv. 5272) | |
| dc.type | info:eu-repo/semantics/article | |
