| dc.contributor | Universidade Estadual Paulista (UNESP) | |
| dc.creator | Alves-Prado, Heloiza Ferreira | |
| dc.creator | Gomes, Eleni | |
| dc.creator | da Silva, Roberto | |
| dc.date | 2014-05-20T15:27:56Z | |
| dc.date | 2016-10-25T18:02:54Z | |
| dc.date | 2014-05-20T15:27:56Z | |
| dc.date | 2016-10-25T18:02:54Z | |
| dc.date | 2007-03-01 | |
| dc.date.accessioned | 2017-04-06T00:04:44Z | |
| dc.date.available | 2017-04-06T00:04:44Z | |
| dc.identifier | Applied Biochemistry and Biotechnology. Totowa: Humana Press Inc., v. 137, p. 41-55, 2007. | |
| dc.identifier | 0273-2289 | |
| dc.identifier | http://hdl.handle.net/11449/37844 | |
| dc.identifier | http://acervodigital.unesp.br/handle/11449/37844 | |
| dc.identifier | 10.1007/s12010-007-9038-2 | |
| dc.identifier | WOS:000246616900006 | |
| dc.identifier | http://dx.doi.org/10.1007/s12010-007-9038-2 | |
| dc.identifier.uri | http://repositorioslatinoamericanos.uchile.cl/handle/2250/881078 | |
| dc.description | A cyclomaltodextrin glucanotransferase (E.C. 2.4.1.19) from a newly isolated alkalophilic and moderately thermophilic Paenibacillus campinasensis strain H69-3 was purified as a homogeneous protein from culture supernatant. Cyclomaltodextrin glucanotransferase was produced during submerged fermentation at 45 degrees C and purified by gel filtration on Sephadex G50 ion exchange using a Q-Sepharose column and ion exchange using a Mono-Q column. The molecular weight of the purified enzyme was 70 kDa by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and the pI was 5.3. The optimum pH for enzyme activity was 6.5, and it was stable in the pH range 6.0-11.5. The optimum temperature was 65 degrees C at pH 6.5, and it was thermally stable up to 60 degrees C without substrate during 1 h in the presence of 10 mm CaCl2. The enzyme activity increased in the presence of Co2+, Ba2+, and Mn2+. Using maltodextrin as substrate, the K-m and K-cat were 1.65 mg/mL and 347.9 mu mol/mg.min, respectively. | |
| dc.language | eng | |
| dc.publisher | Humana Press Inc | |
| dc.relation | Applied Biochemistry and Biotechnology | |
| dc.rights | info:eu-repo/semantics/closedAccess | |
| dc.subject | CGTase characterization | |
| dc.subject | CGTase purification | |
| dc.subject | cyclomaltodextrin glucanotransferase | |
| dc.subject | thermostable CGTase | |
| dc.title | Purification and characterization of a cyclomaltodextrin glucanotransferase from Paenibacillus campinasensis strain H69-3 | |
| dc.type | Otro | |
