THE EFFECT OF SERUM ON IN-VITRO MATURATION, IN-VITRO FERTILIZATION AND STEROIDOGENESIS OF BOVINE OOCYTES COCULTURED WITH GRANULOSA-CELLS

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorMingoti, Gisele Zoccal
dc.creatorGarcia, J. M.
dc.creatorRosaesilva, AAM
dc.date2014-05-20T15:26:29Z
dc.date2016-10-25T18:01:08Z
dc.date2014-05-20T15:26:29Z
dc.date2016-10-25T18:01:08Z
dc.date1995-02-01
dc.date.accessioned2017-04-05T23:57:20Z
dc.date.available2017-04-05T23:57:20Z
dc.identifierBrazilian Journal of Medical and Biological Research. São Paulo: Associação Bras Divulg Cientifica, v. 28, n. 2, p. 213-217, 1995.
dc.identifier0100-879X
dc.identifierhttp://hdl.handle.net/11449/36648
dc.identifierhttp://acervodigital.unesp.br/handle/11449/36648
dc.identifierWOS:A1995QQ60500011
dc.identifier0000-0002-3059-4458
dc.identifierhttp://www.scielo.br/scielo.php?script=sci_issues&pid=0100-879X&lng=en&nrm=iso
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/880131
dc.descriptionThe influence of fetal calf serum alone (FCS) or associated with proestrous (FCS+PCS), estrous (FCS+ECS) or metaestrous (FCS+MCS) cow serum added to the culture medium and of the steroids produced by co-cultured granulosa cells were evaluated in terms of the in vitro maturation (TVM) and fertilization (IVF) of bovine oocytes. Supplementation of the medium with FCS+ECS and FCS+MCS resulted in higher proportions of oocytes that reached metaphase II (96.0% and 93.3%, respectively) and in higher proportions of embryos that reached the four- and eight-cell/morula stages (51.9% and 65.6%, respectively), whereas the supplementation with FCS and FCS+PCS resulted in only 79.2% and 67.5%, respectively, of matured oocytes and 26.7% and 34.3%, respectively, of cleaved embryos. These findings show that the best IVM and IVF were obtained at lower concentrations of estradiol produced by co-cultured granulosa cells (supplementation with FCS+ECS: 10.3 ng/ml and FCS+MCS: 2.1 ng/ml), whereas the worst-results in IVM and IVF occurred at higher concentrations of estradiol that were obtained with FCS (33.1 ng/ml) and FCS+PCS (19.9 ng/ml) supplementation. These data suggest an inhibitory effect of estradiol on resumption of oocyte meiosis in vitro.
dc.languageeng
dc.publisherAssociação Brasileira de Divulgação Científica (ABRADIC)
dc.relationBrazilian Journal of Medical and Biological Research
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectSteroids
dc.subjectIN VITRO MATURATION
dc.subjectIN VITRO FERTILIZATION
dc.subjectOocyte
dc.subjectEmbryo
dc.subjectCattle
dc.titleTHE EFFECT OF SERUM ON IN-VITRO MATURATION, IN-VITRO FERTILIZATION AND STEROIDOGENESIS OF BOVINE OOCYTES COCULTURED WITH GRANULOSA-CELLS
dc.typeOtro


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